Experimental and clinical studies on indication and safety of portal embolization

门脉栓塞适应证及安全性的实验及临床研究

• 批准号: 07671395
• 负责人: YOGITA Shiro
• 金额: $ 1.47万
• 依托单位: The 1st Department of Surgery, The University of Tokushima, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671395/
• 关键词: Portal embolization; AH130; Apoptosis; 門脈枝塞栓術; AH130; ラット

ObjectivesCurrent available treatments for multiple liver cancer are not satisfactory. The aim of this study is to investigate the effects of transcatheter portal embolization (TPE) on the development of experimental liver metastasis in rats.Methods1*10^6 of tumor cells (AH130 hepatoma cell origined from rat) were injected intraportally into Donryu rats to produce liver metastasis 10 days later. All rats (n=24) were divided into four groups and killed 10 days after second operation. 1) Group A ; Control 2) Group B ; TPE of the left branch of portal vein (70% of the total liver) 3) Group C ; left portal ligation 4) Group D ; left hepatic artery ligation. Tumor size, tissue blood flow, regeneration rate of the right lobe, NK activity and immunohistochemical staining of PCNA,apoptosis, VEGF were studied in each group.ResultsPrimarily, hepatic arterial and portal angiography revealed that the tumor had well-developed arterial circulation. Significant hypertrophy of the right lobe and atrop … More hy of the left lobe were observed in Group B and Group C.Rats'weight increased significantly in group B and C,while decreased in Group A and D.Tumor numbers (left/right lobe) were 92.6<plus-minus>23.5/32.3<plus-minus>9.6,88.2<plus-minus>36.2/5.4<plus-minus>2.3,121.3<plus-minus>28.6/6.9<plus-minus>2.1,32.3<plus-minus>5.6/66.0<plus-minus>20.5 in Group A,B,C and D,respectively. Liver blood flow of the left lobe changed from 31.9<plus-minus>6.5 ml/min/100g to 9.0<plus-minus>3.2 in clamp of the main portal vein and to 18.2<plus-minus>4.2 in clamp of the left portal vein branch, suggesting compensatory increase of hepatic artery flow in the left lobe in Group Band C.Expression of VEGF and apoptosis in the tumors did noConclusionTPE resulted in marked hepatic hypertrophy and significant reduction of tumor growth in the non-embolized lobe, probably owing to decrease in arterial flow in the non-embolized lobe. This attractive concept of TPE may have utility in the clinical setting in the event of multiple liver cancer. Less

目的目前治疗多发性肝癌的方法并不令人满意。本研究旨在探讨经导管门静脉栓塞（TPE）对大鼠实验性肝转移发生的影响。方法1*10^6个肿瘤细胞（来源于大鼠的AH130肝癌细胞）腹腔内注射，10 d后产生肝转移。24只大鼠随机分为4组，第二次手术后10 d处死。1) A组；对照组2)B组；门静脉左支TPE（占全肝70%）3)C组；左门静脉结扎术4)D组；左肝动脉结扎。观察各组肿瘤大小、组织血流量、右肺叶再生率、NK活性及PCNA、凋亡、VEGF的免疫组化染色。结果肝动脉及门静脉造影显示肿瘤动脉循环发达。B、C组大鼠体重明显增加，A、D组大鼠体重明显减少。A、B、C、D组大鼠左右叶肿瘤数分别为92.6<正负>23.5/32.3<正负>9.6、88.2<正负>36.2/5.4<正负> 2.31.3 <正负>28.6/6.9<正负>2.1、32.3<正负>5.6/66.0<正负>20.5。左叶肝血流量的变化从31.9 <加减> 6.5毫升/分钟/ 100克9.0 <加减> 3.2夹的主要门静脉和18.2 <加减> 4.2夹左门静脉分支,建议补充增加左叶肝动脉流组乐队C.Expression VEGF和细胞凋亡在肿瘤noConclusionTPE导致肝肥大和肿瘤生长的显著减少non-embolized叶,可能是由于未栓塞叶动脉血流减少所致。这个有吸引力的TPE概念可能在多发性肝癌的临床环境中有实用价值。少

项目成果

Yogita S: "Hepatocellular carcinoma with extension into the right atrium : report of a successful liver resection by hepatic vascular exclusion exclusion using cardiopulmonary bypass" 2nd World Congress International Hepato-Biliary Association Proceedings

Yogita S：“肝细胞癌扩散至右心房：通过体外循环肝血管排除成功切除肝脏的报告”第二届世界大会国际肝胆协会会议记录

S.YOGITA: "Hepatocellular carcinoma with extension into the right atrium:report of a successful liver resection by hepatic vascular exclusion using cardiopul monary bypass" 2nd World Congress International Hepato-Pancreato-Biliary Association. 337-340 (19

S.YOGITA：“肝细胞癌扩散至右心房：使用体外循环肝血管阻断术成功切除肝脏的报告”第二届国际肝胰胆协会世界大会。

Yogita S.: "A study on the modes of recurrence after hepatectomy for hepatocellular carcinoma and the clinical outcomes" Journal of Japanese Society for Clinical Surgery. 58. 28-33 (1997)

Yogita S.：“肝细胞癌肝切除术后复发模式及其临床结果的研究”日本临床外科学会杂志。

S.YOGITA: "Hepatocellular carcinoma with extension into the right atrium:report of a successful liver resection by hepatic vascular exclusion using cardiopulmonary bypass" 2nd World Congress International Hepato-Pancreato-Biliary Asscciation. 337-340 (199

S.YOGITA：“肝细胞癌扩散至右心房：使用体外循环肝血管阻断术成功切除肝脏的报告”第二届国际肝胰胆协会世界大会。

余喜多史郎: "肝細胞癌切除後再発様式と臨床経過の検討" 日本臨床外科医学会雑誌. 58・1. 28-33 (1997)

横田四郎：“肝细胞癌切除后的复发模式和临床过程的检查”日本临床外科医师学会杂志58・1（1997）。