脳血管攣縮の平滑筋細胞障害におけるアポトーシスの関与とサイトカインの効果の研究

测定犬基底动脉细胞内钙水平，以研究蛛网膜下腔出血后慢性血管痉挛期间其平滑肌的钙调节是否改变。采用双出血模型。血管痉挛的发生在首次出血后7天通过血管造影证实。Fura-2测定平滑肌细胞内钙离子浓度（[Ca 2 +] i）。监测在340和356 nm处的荧光与激发，并将比率R340/356用作[Ca 2 +] i的指示剂。当细胞外钙离子浓度（[Ca ~（2+）] e）由pCa ~（8）增加到pCa ~（2+）2时，[Ca ~（2+）] i也增加。痉挛动脉的[Ca ~（2+）]_e- [Ca ~（2+）]_i曲线较正常动脉增高。离子霉素治疗使正常组的曲线升高，但对痉挛动脉影响不大。[Ca 2 +] i的值，计算的Kd的倍数，在痉挛的动脉更大。地尔硫卓（10 ~（-5）mol/l）可部分抑制痉挛动脉[Ca ~（2+）] i信号的增强，而对对照组的曲线无影响。提示蛛网膜下腔出血后平滑肌钙调节功能受损，可能参与了慢性血管痉挛的发病机制。

The intracellular calcium level was determined in the canine basilar artery in order to investigate whether Ca2+ regulation of its smooth muscle is altered during chronic vasospasm following subarachnoid hemorrhage. A double hemorrhage model was used. The occurrence of vasospasm was confirmed angiographically seven days after initial hemorrhage. The intracellular calcium concentration ( [Ca2+] i) of smooth muscle was measured using Fura-2. Fluorescence to excitation at 340 and 356 nm was monitored and the ratio R340/356 was used as the indicator of [Ca2+] i. When the extracellular calcium concentration ( [Ca2+] e) was increased from pCa 8 to 2, [Ca2+] i also increased. In the spastic arteries, the [Ca2+] e- [Ca2+] i curve was elevated as compared to the normal rteries. Treatment with ionomycin elevated the curve in the normal group, but it had little effect in the spastic arteries. Values of [Ca2+] i, calculated in multiples of Kd, were greater in the spastic arteries. Diltiazem (10-5 mol/l) partially suppressed the augmented [Ca2+] i signal in the spastic arteries, whereas it did not affect the curve in the control group. These results indicate that calcium regulation of smooth muscle impaired after subarachnoid hemorrhage, which may contribute to the pathogenesis of chronic vasospasm.