Development of a Novel and Reliable Model for Subarachnoid Hemorrhage and Vasospasm in Rats

开发一种新颖可靠的大鼠蛛网膜下腔出血和血管痉挛模型

• 批准号: 12671378
• 负责人: KIM Phyo
• 金额: $ 2.18万
• 依托单位: Dokkyo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671378/
• 关键词: Subarachnoid hemorrhage; Cerebral Vasospasm; Rat; Stereotaxis; 血管攣縮; 時間経過

A novel rat SAH model was devised and reproducible induction CVS was observed. The temporal profile of delayed onset and prolonged duration, as well as its refractoriness to conventional pharmacological treatments are the hallmarks of cerebral arterial spasm following subarachnoid hemorrhage. Morphological changes of the artery was not evident in our model. Changes such as damages of the endothelium, subendothelial proliferation, necrosis of the smooth muscle have been described in clinical specimens and in the experimental models, but there is no unanimous agreement on their true significance. Focal neurologic deficits are not observed in our spasm model despite severe arterial constriction, presumably due to abundance in the collateral circulation. The stereotactic technique for induction of hemorrahge is very simple and suitable for conducting a large number of experiments such as in therapeutics trial with pharmacological agents. Although duration of the chronic spasm in the model is short, typically reaching maximum on the second day, the intensity of the spasm is severe and the phenomenon of the clot (hemoglobin)-artery (smooth muscle) interaction is reliably reproduced.

设计了一种新型的大鼠SAH模型，并观察到可重复的诱导CVS。延迟发作和延长持续时间的时间特征及其对常规药理治疗的耐磨性是亚蛛网膜下腔出血后脑动脉痉挛的标志。在我们的模型中，动脉的形态变化并不明显。在临床标本和实验模型中已经描述了内皮损害，内皮下皮增殖，平滑肌坏死的变化，但在其真正意义上没有一致的一致一致。尽管有严重的动脉收缩，但在我们的痉挛模型中未观察到局灶性神经系统缺陷，这可能是由于附带循环的丰富性。诱导出血的立体定向技术非常简单，适合进行大量实验，例如用药理学剂进行治疗试验。尽管模型中慢性痉挛的持续时间很短，通常在第二天达到最大值，但痉挛的强度很严重，并且凝块（血红蛋白） - artery（平滑肌）相互作用的现象可靠地重现。