Biomechanical analyzes on effects of change of initial tension on the mechanical properties of the frozen anterior cruciate ligament in the dog.

初始张力变化对狗冷冻前十字韧带力学性能影响的生物力学分析。

• 批准号: 07671557
• 负责人: MIYAGI Noboru
• 金额: $ 1.66万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671557/
• 关键词: anterior cruciate ligament; freezing; initial tension; biomechanics; mechanical property; fibroblast; tensile strength; ultrastructure; 靱帯再建; 引っ張り強度; 自家腱移植

1. We developed helical cryoprobe in which liquid nitrogen was circulated to perform in situ freezing of the canine anterior cruciate ligament (ACL). To know the ability of the cryoprobe we froze the ACLs of 12 adult dogs. After freezing, the ligaments were investigated with microscope and cell culture to detect living cell. No living cell was found in the frozen ACL.It was proved that the in situ freezing killed all fibroblasts of the ACL.2. The ACLs of the 20 mature beagle dogs were frozen in situ with the specially designed helical cryoprobe. After all fibroblasts died, the ACLs were thawed out with physiological saline. The tibial insertion of each ACL was made free from the tibia with a cylindrical bone block by penetrating a hollow reamer. The dogs were divided in two groups. At the first group, the bone block was reduced anatomically (physiological tension group : P group). At the second group, an initial tension of 20 N was applied on the ACL by pulling the bone block to the di … More stal direction with a tensiometer, resulting in distal translocation of the tibial insertion (high tension group : H group). Then, the each bone block was firmly fixed with an interference screw. The differences of the in situ frozen ACLs between the P and the H groups were investigated about the changes of mechanical properties and ultrastructure.3. There were no significant differences in the mechanical properties between the P and the H groups at 6 weeks after surgery. On the other hand, the tensile strength, maximum load and the tangent modulus in the H group were significantly lower than those in the P group at 12 weeks (p<.05). No significant differences could be found in thecross sectional area and in the strain at failure.4. The ACLs of four dogs were analyzed ultrastructurally. At 6 weeks after surgery, fibroblasts entered into the superficial substance of the in situ frozen ACL in the P group, although inflammatory cells entered into the intersubstancial space in the H group. At 12 weeks, the fibroblasts were found at all the substance of the ACL in the P group, although the degenerative change of the substance was found in the H group. Less

1.我们开发了螺旋冷冻探针，液氮循环，对犬前交叉韧带（ACL）进行原位冷冻。为了了解冷冻探针的能力，我们冷冻了 12 只成年狗的 ACL。冷冻后，用显微镜和细胞培养物对韧带进行研究以检测活细胞。冷冻后的ACL中未发现活细胞，证明原位冷冻杀死了ACL的全部成纤维细胞。2．使用专门设计的螺旋冷冻探针对 20 只成年比格犬的 ACL 进行原位冷冻。所有成纤维细胞死亡后，用生理盐水解冻ACL。通过穿透空心铰刀，用圆柱形骨块使每个 ACL 的胫骨插入脱离胫骨。这些狗被分成两组。在第一组中，骨块在解剖学上减少（生理张力组：P组）。在第二组中，通过用张力计将骨块向远端方向拉动，在 ACL 上施加 20 N 的初始张力，导致胫骨插入的远端易位（高张力组：H 组）。然后，用干涉螺钉将每个骨块牢固地固定。考察P组和H组原位冷冻ACL力学性能和超微结构变化的差异。 3．术后6周，P组和H组的力学性能无显着差异。另一方面，12周时H组的拉伸强度、最大载荷和切线模量显着低于P组(p<.05)。横截面积和失效应变没有发现显着差异。4．对四只狗的 ACL 进行超微结构分析。术后6周，P组成纤维细胞进入原位冷冻ACL的表层实质，而H组炎症细胞进入实质间隙。 12周时，P组ACL全部物质均可见成纤维细胞，H组则有物质退行性改变。较少的

项目成果

Yasuda K: "Effects of initial graft tension on clinical outcome after anterior cruciate ligament reconstruction." Am. J. Sports Med.25. 99-106 (1997)

Yasuda K：“初始移植物张力对前交叉韧带重建后临床结果的影响。”

Miyata K,Yasuda K,Kimura S,Hara N,Kaneda K: "Measurement of the mechanical properties of the porcine patellar and idgital flexor tendons and verification of an anterior cruciate ligament reconstruction model using their tendons (Japanese text)." J Jpn Soc

Miyata K、Yasuda K、Kimura S、Hara N、Kaneda K：“测量猪髌骨和髂屈肌腱的机械性能，并使用其肌腱验证前十字韧带重建模型（日语文本）。”

Tohyama H: "Inhibition of extrinsic cell infiltration prohibits reduction of the mechanical properties of the patellar tendon following intrinsic cell necrosis." Trans. Orthop. Res. Soc.21. 29-29 (1996)

Tohyama H：“抑制外在细胞浸润可以防止内在细胞坏死后髌腱机械性能的降低。”

宮田康史: "ブタ膝蓋腱および深指屈筋腱の力学的特性とこれを用いた前十字靱帯再建モデルの検討" 日本臨床バイオメカニクス学会誌. 17. 427-431 (1996)

Yasushi Miyata：“猪髌腱和指深屈肌腱的力学特性以及使用它们的前交叉韧带重建模型的研究”日本临床生物力学学会杂志 17. 427-431 (1996)。

葛城 良成: "過負荷が凍結処理した犬の前十字靱帯の力学的特性に与える効果" 日本臨床バイオメカニクス学会誌. 17(印刷中). (1996)

Yoshinari Katsuragi：“超负荷对冷冻治疗犬前十字韧带机械性能的影响”，日本临床生物力学学会杂志 17（出版中）。