Fast Freezing Processes in Tissues

组织中的快速冷冻过程

基本信息

  • 批准号:
    10798699
  • 负责人:
  • 金额:
    $ 8.53万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-06-01 至 2024-05-31
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY (ORIGINAL) The goal of the research is to advance the formulation of cryopreservation protocols for biological systems using fundamental biophysical parameters. At present, crucial gaps exist in our knowledge of the biophysical parameters governing the states of intra- and extra-cellular water during a fast freezing process. This proposal aims to redress this crucial gap by introducing two new and effective techniques: (a) a measurement technique based on microscale thermoelectric sensors, engineered using microfabrication techniques suitable for use with artificial tissues and (b) a measurement technique based on a combination of calorimetry and low temperature microscopy suitable for use with native tissues. Specifically, the project will: (1) design fabricate and test microscale thermoelectric sensors to measure fast freezing processes in isolated cells (Neonatal Human Dermal fibroblasts, NHDFs) and artificial tissues (NHDFs embedded in a collagen gel sheet) with and without Cryoprotective Agents (CPAs) and (2) develop a combination of calorimetric and low temperature microscopy (directional solidification) methods to assess fast freezing processes in NHDFs and native (mammalian liver) tissue sections with and without CPAs. CPA addition and removal in both tissue (artificial and native) systems will be modeled using irreversible thermodynamic models. These integrated methods from disparate disciplines will lead to rational design and development of freezing processes for cryopreservation of donated tissues, an extremely scarce resource, as well as artificially engineered tissues. The education and infrastructure development part of this NIH AREA 15 proposal includes both instructional and research components for LSU undergraduate students. PROJECT SUMMARY (NIGMS Supplemental Award) The NIGMS supplemental (equipment) award requests funds to purchase a state of the art differential scanning calorimeter (DSC 8500) to enhance the quality of the data obtained in Aim 2 of the project. The new instrument will allow us to limit measurement errors and to assess the true contribution of cell heterogeneity. In addition, the new instrument will controllably freeze cells to a much higher rates (~750 ˚C/min) thus allowing us to fully accomplish the goals of the original proposal, i.e., to assess fast freezing processes in tissues.
项目总结(原件)

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Freezing of Solute-Laden Aqueous Solutions: Kinetics of Crystallization and Heat- and Mass-Transfer-Limited Model.
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Ram Devireddy其他文献

Ram Devireddy的其他文献

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{{ truncateString('Ram Devireddy', 18)}}的其他基金

Fast Freezing Processes in Tissues
组织中的快速冷冻过程
  • 批准号:
    10200316
  • 财政年份:
    2021
  • 资助金额:
    $ 8.53万
  • 项目类别:
Assessing a Novel Paradigm of Inducing Cryotolerance
评估诱导低温耐受的新范式
  • 批准号:
    8243062
  • 财政年份:
    2012
  • 资助金额:
    $ 8.53万
  • 项目类别:
Assessing a Novel Paradigm of Inducing Cryotolerance
评估诱导低温耐受的新范式
  • 批准号:
    8441521
  • 财政年份:
    2012
  • 资助金额:
    $ 8.53万
  • 项目类别:
Ice Nucelation in Biological Tissues - Implications to Cryopreservation
生物组织中的冰成核 - 对冷冻保存的影响
  • 批准号:
    7277323
  • 财政年份:
    2006
  • 资助金额:
    $ 8.53万
  • 项目类别:
Ice Nucelation in Biological Tissues - Implications to Cryopreservation
生物组织中的冰成核 - 对冷冻保存的影响
  • 批准号:
    7142815
  • 财政年份:
    2006
  • 资助金额:
    $ 8.53万
  • 项目类别:

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