Cell Biological Study for the Mechanism of Osteogenesis by Electromagnetic Fields.

电磁场成骨机制的细胞生物学研究。

• 批准号: 07671606
• 负责人: MATSUNAGA Shunji
• 金额: $ 1.47万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671606/
• 关键词: Electromagnetic; Osteogenesis; Ca ion; intra cellular signaling; Osteoblast; 副甲状腺ホルモン

The following cxperimental results were obtained by this research.1.Changes in Ca-ion incorporation into cells by stimulation of electromagnetic field in vitroIntracellular Ca-ion concentration in osteoblasts that underwent electromagnetic field stimulation was higher the that in control osteoblasts by 15% on average. This acceleration of Ca-ion incorporation was most efficient in the electromagnetic field of 100 Hz in frequency, 15 (sec in pulse width and 2.0 gauss of the magnetic field intensity.2.Evaluation of in vivo osteogenesis-enhancing effect of electromagnetic field stimulation at cellular levelOsteogenesis measured by an osteometer significantly increased under stimulation by the electromagnetic field of 100 Hz in frequency, 15 (sec in pulse width and 2.0 gauss of the magnetic field intensity. These results reflected those obtained in the in vitro experiment in the present study. In the examination of activity of alkaline phosphatase, a marker of osteoblast, and proliferation of osteoblasts using AgNOR staining, elevation of the enzyme and enhancement of proliferation were shown under the optimum stimulation conditions of the electromagnetic field.As the mechanism of enhancement of osteogenesis by stimulation with the electromagnetic field, these results demonstrated that Ca-ion uptake by cells was changed by the electromagnetic field stimulation, changes in functions at the cellular level such as synthesis of alkaline phosphatase and cell-proliferation were induced via intracellular signal transaction in osteoblasts, and osteogenesis was enhanced. The fact that osteogenesis, and growth and Ca-ion incorporation by osteoblasts were changed by conditions of electromagnetic field stimulation strongly suggests the effect of electromagnetic field on cells.

本研究得到的实验结果如下：体外电磁场刺激下细胞内钙离子掺入的变化经电磁场刺激的成骨细胞细胞内钙离子浓度比对照成骨细胞平均高15%。在频率为100 Hz、脉宽为15秒、磁场强度为2.0高斯的电磁场中，ca离子掺入的加速效果最好。在频率为100 Hz、脉冲宽度为15秒、磁场强度为2.0高斯的电磁场刺激下，骨计测量的成骨量显著增加。这些结果反映了本研究中体外实验的结果。用AgNOR染色法检测成骨细胞标志物碱性磷酸酶的活性和成骨细胞的增殖情况，发现在最佳电磁场刺激条件下，碱性磷酸酶活性升高，增殖增强。作为电磁场刺激促进成骨的机制，这些结果表明，电磁场刺激改变了细胞对钙离子的摄取，通过细胞内信号交换诱导成骨细胞碱性磷酸酶合成和细胞增殖等细胞水平功能的改变，促进了成骨。电磁场刺激条件下成骨细胞的成骨、生长和钙离子掺入均发生改变，说明电磁场对细胞有一定的影响。

项目成果

Kouji Yonemori: "The effect of electrical stimulation on osteogenesis" Bone. 19. 173-180 (1996)

Kouji Yonemori：“电刺激对成骨的影响”骨。

Kosei Ijiri: "The effect of pulsing electromagnetic field on bone ingrowth into porous coated implant." Anticancer Research. 16. 2853-2856 (1996)

Kosei Ijiri：“脉冲电磁场​​对多孔涂层植入物中骨骼长入的影响。”

Shunji Mastunaga: "Osteogenesis by pulsing electromagnetic Fields(PEMFs):Optimum stimulation setting." in vivo. 10. 351-356 (1996)

Shunji Mastunaga：“脉冲电磁场​​（PEMF）成骨：最佳刺激设置。”

Kouji Yonemori: "The effect of electrical stimulation on osteogenesis." Bone. 19. 173-180 (1996)

Kouji Yonemori：“电刺激对成骨的影响。”

Shunji Matsunaga: "Osteogenesis by pulsing electromagnetic Fields (PEMFs) : Optimum stimulation setting." in vivo. 10. 351-356 (1996)

Shunji Matsunaga：“脉冲电磁场​​ (PEMF) 成骨：最佳刺激设置。”