Uptake and elimination of sevoflurane in rabbit tissues-An in vivo 19F magnetic resonance study

兔组织对七氟醚的摄取和消除——体内 19F 磁共振研究

• 批准号: 07671646
• 负责人: MAKITA Koshi
• 金额: $ 1.22万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671646/
• 关键词: sevoflurane; pharmacokinetics; magnetic resonance spectroscopy; セボフルレン

Background : An in vivo pharmacokinetic study of fluorinated inhalational anesthetics is possible using ^<19>F magnetic resonance spectroscopy. Previous pharmacokinetic studies were performed mainly in brain as it is considered as a site of anesthetic action. Investigation of other tissues will give us more precise informations of the pharmacokinetics of inhalatlonal anesthetics in a whole body. In this study we investigated the pharmacokinetics of uptake and elimination of sevoflurane in brain, liver, muscle, venous blood and arterial blood of rabbits using in vivo 19F MRS in order to compare differences of pharmacokinetics among tissues and also differences between uptake and elimination in each tissue. Methods : In twenty two rabbits 19F magnetic resonance spectroscopy was conducted at 4.7 Tesla using a 1 cm diameter surface coil for brain (n-4), liver (n-5) and muscle (n-5), and a 1.3 cm diameter surface coil for arterial (n-4) and venous (n=4) blood.Results : Sevoflurane was administered for 120 min, followed by a 120-min period of elimination. Both uptake and elimination kinetics were best fit to a biexponential curve which was divided into the fast and slow components. During an uptake experiment times to reach half of the maximum spectroscopic intensity in each tissue were 1.5 min in arterial blood, 4.5 min in liver, 12.7 min in brain, 13.1 min in venous blood and 27.3 min in muscle. During an elimination experiment times to reach half of the maximum intensity in each tissue were 2.4 min in arterial blood, 5.5 min in liver, 12.5 min in venous blood, 20.4 min in brain and 26.7 min In muscle.Conclusions : Sevoflurane uptake or elimination in each tissue was able to be expressed by biexponential kinetics. Sevoflurane uptake and elimination kinetics in arterial blood were rapid and almost similar to end-tidal sevoflurane kinetics. Those in liver were next fastest followed by brain, venous blood and muscle.

背景：氟吸入麻醉药的体内药代动力学研究是可能的，使用13 <19>F磁共振波谱。既往药代动力学研究主要在脑中进行，因为脑被视为麻醉作用部位。对其他组织的研究将为我们提供吸入麻醉药在全身的药代动力学更精确的信息。在本研究中，我们使用体内19 F MRS研究了七氟烷在家兔脑、肝脏、肌肉、静脉血和动脉血中的摄取和消除的药代动力学，以比较组织间的药代动力学差异以及各组织中摄取和消除的差异。研究方法：在22只家兔中，在4.7特斯拉下使用直径为1 cm的表面线圈对脑（n-4）、肝（n-5）和肌肉（n-5）进行19 F磁共振波谱分析，并使用直径为1.3 cm的表面线圈对动脉（n-4）和静脉（n=4）血液进行19 F磁共振波谱分析。吸收和消除动力学最佳拟合为双指数曲线，该曲线分为快速和缓慢分量。在摄取实验期间，在每个组织中达到最大光谱强度的一半的时间在动脉血中为1.5分钟，在肝脏中为4.5分钟，在脑中为12.7分钟，在静脉血中为13.1分钟，在肌肉中为27.3分钟。在消除实验中，各组织中达到最大强度一半的时间分别为动脉血2.4 min、肝脏5.5 min、静脉血12.5 min、脑20.4 min和肌肉26.7 min。动脉血中七氟烷的摄取和消除动力学迅速，几乎与潮气末七氟烷动力学相似。肝脏中的那些是第二快的，其次是大脑，静脉血和肌肉。

项目成果

槇田 浩史: "ウサギ急性肺傷害における部分的液体換気における酸素化能改善とパーフルブロン分布の関係-磁気共鳴画像による研究" 日本集中治療医学会雑誌. (in press). (1999)

Hiroshi Makita：“兔子急性肺损伤部分液体通气期间改善的氧合能力与 perflubron 分布之间的关系 - 使用磁共振成像的研究”日本重症监护医学会杂志（1999 年出版）。