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Influences of angiogenic factors affecting growth and progression of the bladder cancer.

血管生成因子对膀胱癌生长和进展的影响。

基本信息

批准号：
07671706
负责人：
SATO Kazunari
金额：
$ 1.47万
依托单位：
Akita University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

1) Expression of VEGF and its receptor genes in transitional cell carcinoma (TCC) of the bladder.VEGF transcripts and its product were observed in all of the tumors. The VEGF gene expression of invasive TCCs was significantly higher than that of superficial TCCs (p<0.05). All of the tumors as well as the normal mucosae and HUVEC-1 cells expressed flt-1 gene, while WBCs of patients did not. The expression level of flt-1 gene was significantly correlated with that of VEGF gene in the tumor. These results have demonstrated that the VEGF are frequently overexpressed in TCC,especially in muscle invasive tumors, and a paracrine system exists between the TCC cells and the adjacent endothelial cells, regulating the angiogenesis in the TCC.2) Regulation of VEGF gene expression in TCC.Aberrations of p53 gene was detected in 38.7% of urothelial carcinomas and had a tendency to be dominant in high grade and high stage tumors. The VEGF gene expression of muscular invasive urothelial carcinomas was … More higher than those of normal mucosae and superficial tumors (p<0.01). There was, however, no significant difference in VEGF expression between the tumors with p53 gene aberration and the tumors without it. These results suggest that both VEGF and p53 genes play important roles in malignant potential of the urothelial carcinoma. However, VEGF gene expression is regulated by a mechanism other than p53 in this tumor.3) Angiogenic factors and microvessels in TCC.Positive rate of VEGF,bFGF and aFGF were 50%, 23% and 43% in the superficial bladder cancer, respectively. T1 tumors had a higher maximum vessel density than Ta. Microvessels were highlighted by staining for factor VIII-related antigen. Average vessel density and vascular surface density of the tumor were higher than those of normal epithelium. Significant negative correlation between the number of angigenic factors expressed and vessel density was found. These findings suggest that the expression of angiogenic factors correlates with malignant transformation of urothelium, angiogenesis is required topically at the invasive front of the superficial bladder cancer.Summary ; Angiogenic factors, especially VEGF,play important roles in transformation and progression as well as angiogenesis of this tumor Less

1)VEGF及其受体基因在膀胱移行细胞癌（TCC）中的表达：所有肿瘤中均检测到VEGF转录本及其产物。侵袭性膀胱移行细胞癌VEGF基因表达显著高于浅表性膀胱移行细胞癌（p<0.05）。所有肿瘤组织、正常粘膜组织和HUVEC-1细胞均表达flt-1基因，而患者白细胞均不表达。flt-1基因与VEGF基因在肿瘤中的表达水平呈显著相关。这些结果表明，VEGF在TCC中经常过表达，特别是在肌肉浸润性肿瘤中，并且在TCC细胞和邻近的内皮细胞之间存在旁分泌系统，2）VEGF基因在膀胱移行细胞癌中表达的调节。p53基因在膀胱移行细胞癌中的表达异常率为38.7%，在高级别、高分期肿瘤中有优势表达的趋势。VEGF基因在肌层浸润性尿路上皮癌中的表达， ...更多信息高于正常粘膜和浅表肿瘤（P<0.01）。p53基因异常组与正常组VEGF表达无显著性差异，提示VEGF和p53基因在尿路上皮癌的恶性潜能中起重要作用。3）膀胱移行细胞癌中VEGF、bFGF和aFGF的阳性表达率分别为50%、23%和43%。T1肿瘤的最大血管密度高于Ta。通过因子VIII相关抗原染色突出显示微血管。肿瘤的平均血管密度和血管面密度均高于正常上皮。血管生成因子的表达数量与血管密度呈显著负相关，提示血管生成因子的表达与膀胱癌的恶性转化有关，血管生成是浅表性膀胱癌浸润前沿局部所必需的。