Prevalence of Human Papillomavirus in Cervical Cancer in Okinawa

冲绳人乳头瘤病毒在宫颈癌中的患病率

• 批准号: 07671809
• 负责人: HIGASHI Masahiro
• 金额: $ 1.47万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671809/
• 关键词: human papillomavirus; cervical cancer; metastatic lymphadenopathy; 子宮頚部異形成; 子宮頸癌罹患率; NPV感染; 子宮頸部異形成

1)Prevalence of human papillomavirus (HPV) in the screening smear test for cervical cancer in OkinawaHPV infection has now been shown to be the primary cause of most cases of cervical intraepithelial neoplasia and is a necessary, but not sufficient, cause of most cases of cervical cancer. HPV testing relies on the detection of viral deoxyribonucleic acid (DNA) in exfoliated cell specimens.We examined the correlation between the progression of cervical dysplasia and the prevalence of HPV by means of the polymerase chain reaction (PCR).The PCR with L1 consensus primers could identify at least nine HPV types, 6,11,16,18,31,33,42,52 and 58 (6), and the detailed typing of HPVs is needed in our study.Cervical scrapes were collected from 3,985 women who received the screening smear test for cervical cancer in Okinawa.The prevalence of HPVs in normal cervices (Pap class I and II) was that under 30 years old : 18.7% (45/241), 31-40 years old : 8.0% (47/587), 41-50yrs : 9.3% (76/817), 51-60yrs : … More 10.0% (106/1059), 61-70yrs : 10.2% (104/1018) and over 70yrs : 9.8% (26/263).At present, we are investigating, prospectively, the prevalence of HPV DNA after treatment and the predictive value of HPV positivity for recurrence of abnormal cytology and cervical intraepithelial neoplasias (CIN). Our study indicates that detectability of HPV DNA does coincide with detectability of CIN and that effective surgical treatment of CIN can also eliminated detectable presence of HPV DNA.2)Clinical significance of human papillomavirus detection from metastasis-suspected tissues in cervical cancer.Studies on Human papillomaviruses (HPVs) are prevalent in primary diseases of cervical cancer. The vast majority of these studies have been done using the technique to detect HPV DNAs in paraffin-embedded tissues. On the other hand, only limited data are available on the detection of HPV DNAs in metastatic lesions.The aim of the present study was to establish the procedure of DNA extraction from fresh tissue specimens for HPV analysis and to examine the correlation between the presence of HPVs and pathological evidence in metastasis-suspected tissues.CONCLUSION1.HPV DNAs were detectable in cell samples prepared from fresh metastasis-suspected tissues as well as primary cervical lesions.2.HPV type in metastatic lesions was identical with that in their primary cervical lesions in all of HPV-positive patients.3.Of 6 cases with HPV-positive nodes not histologically proven to be metastasized, 3 suffered from recurrence a few months later at the sites where the HPV-positive nodes were located, suggesting that the nodes had been already metastasized at the time of sampling.4.HPV-positive detection in metastasis-suspected tissues could provide early information for the appraisal of possible metastasis. Less

1)宫颈癌筛查涂片检查中人乳头瘤病毒（HPV）的感染率现已被证明是大多数宫颈上皮内瘤变病例的主要原因，也是大多数宫颈癌病例的必要但不充分的原因。HPV检测依赖于脱落细胞标本中病毒脱氧核糖核酸（DNA）的检测。我们通过聚合酶链反应（PCR）检测了宫颈不典型增生的进展与HPV患病率的相关性。使用L1共有引物的PCR可以识别至少9种HPV类型，6、11、16、18、31、33、42、52和58（6），收集了冲绳3，985名接受宫颈癌筛查涂片检查的妇女的宫颈刮片，（巴氏Ⅰ和Ⅱ级）为30岁以下：18.7%（45/241），31-40岁：8.0%（47/587），41- 50岁：9.3%（76/817），51- 60岁： ...更多信息 10.0%（106/1059），61- 70岁：10.2%（104/1018），70岁以上：9.8%（26/263）。目前，我们正在前瞻性地调查治疗后HPV DNA的患病率以及HPV阳性对异常细胞学和宫颈上皮内瘤变（CIN）复发的预测价值。本研究提示HPV DNA的检出率与CIN的检出率一致，CIN的有效手术治疗也可消除HPV DNA的检出。2）宫颈癌可疑转移组织中HPV检测的临床意义这些研究中的绝大多数都是使用该技术在石蜡包埋组织中检测HPV DNA。另一方面，在一项研究中，转移灶中HPV DNA的检测资料有限，本研究旨在建立从新鲜组织标本中提取DNA用于HPV分析的方法，并研究HPV的存在与可疑转移组织病理学证据之间的相关性。HPV阳性者转移灶与原发灶HPV型别一致，6例组织学未证实HPV阳性淋巴结转移者，3例数月后HPV阳性淋巴结复发，提示在取样时淋巴结已经发生转移。4.在疑似转移组织中检测HPV阳性可为评估可能的转移提供早期信息。少

项目成果

Maehara T,Patzelt A,Hutter K,Kanazawa K,Zur Hausen H,Rosl F.: "Selective downregulation of human papilloma virus transcription by 2-desoxyglucose." Int.J.Cancer. (in press).

Maehara T、Patzelt A、Hutter K、Kanazawa K、Zur Hausen H、Rosl F.：“2-脱氧葡萄糖选择性下调人乳头状瘤病毒转录。”

T.Asato, et al: "Correlation between the Progression of Cervical Dysplasia and the Prevalence of Human Papillomavirus" THE JOURNAL OF INFECTIOUS DISEASES.169. 940-941 (1994)

T.Asato 等人：“宫颈发育不良的进展与人乳头瘤病毒流行之间的相关性”传染病杂志.169。

長井 裕、他: "子宮頚癌の転移診断におけるHPV DNA検出の意義" 日本産科婦人科学会雑誌. 48. S465 (1996)

Yutaka Nagai等人：“HPV DNA检测在诊断宫颈癌转移中的意义”日本妇产科学会杂志48.S465(1996)。

長井 裕、他: "子宮頚癌の転移診断におけるHPV DNA検出の意義" 日本産科婦人科学会雑誌. 48. S465- (1996)

Yutaka Nagai等人：“HPV DNA检测在诊断宫颈癌转移中的意义”日本妇产科学会杂志48.S465-(1996)。

Maehama,T.,et al: "Selective down-regulation of human papilloma virus transcription by 2-deoxyglucose." Int.J.Cancer,. (in press).

Maehama,T.,et al：“2-脱氧葡萄糖选择性下调人乳头状瘤病毒转录。”