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Pregnancy and cell adhesion molecules ; Role of cell adhesion molecules in the pathogenesis of spontaneous abortion and preeclampsia

妊娠与细胞粘附分子；

基本信息

批准号：
07671831
负责人：
TAKESHITA Toshiyuki
金额：
$ 0.13万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671831/
关键词：
abortion preeclampsia NK cell cell adhesion molecules feto-mateal immune response ICAM-1 IUGR 免疫

项目摘要

1.Preeclampsia and Cell Adhesion MoleculesVascular endothelial cell (EC) injury is thought to play a central role in the pathogenesis of preeclampsia. Natural killer cells might be one of the possible effectors targeting endothelial cells as well as neutrophils, macrophages and cytotoxic T-Iymphocytes. It has been reported that cell adhesion molecule, ICAM-1 and IFA-1 could be involved in NK cell adhesion to ECs. These evidences allowed us to investigate the interaction between NK cells and vascular ECs in the pathogenesis of pre-eclampsia. NK cell activity in preeclamptic women with severe proteinuria found to be significantly augmented. NK cell activity was also augmented in pre-eclamptic women with IUGR.Endothelial cells (ECs) were incubated overnight in the presence of patient sera with severe preeclampsia. ICAM-1 expression on ECs was clearly enhanced when cultured in medium containing patient sera. These results suggest that there is a factor which enhance the expression of ICAM- … More 1 on the cell surface of ECs and that the interaction between NK cells and ECs might be involved in the pathogenesis of pre-eclampsia.2.Spontaneous abortion and Cell Adhesion MoleculesTransplanted heart graft survives in the animals injected with anti-ICAM-1 antibody and anti-LFA-I antibody The spontaneous abortion is regarded as a rejection of semiallograft by maternal immune system. We investigated an efficacy of the injection of anti-adhesion molecule antibody to prevent fetal resorptions in the murine spontaneous miscarriage model, CBAXDBA/2 matings. CBA/J female mice mated with DBA/2 male mice were injected intraperitoneally with anti-ICAM-1 antibody and anti-LFA-I antibody for 5 days from day I to 5 of gestation. On day 13 of gestation, pregnant mice were sacrificed and viable and resorbing embryos were counted. Although there was not a significant difference between the number of implantation sites for antibody injected group and that for control (saline injected) group, the number of living embryo was significantly increased in antibody treated group. These results suggest that spontaneous fetal resorption can be prevented by controlling cellular interaction mediated by cell adhesion molecules. Less

1.子痫前期与细胞粘附分子血管内皮细胞（EC）损伤被认为在子痫前期的发病机制中起重要作用。自然杀伤细胞可能是靶向内皮细胞以及中性粒细胞、巨噬细胞和细胞毒性T淋巴细胞的效应细胞之一。已有研究表明，细胞粘附分子ICAM-1和IFA-1参与了NK细胞与内皮细胞的粘附。这些证据使我们能够研究NK细胞和血管内皮细胞之间的相互作用在先兆子痫的发病机制。NK细胞活性在重度蛋白尿的先兆子痫妇女被发现显着增强。NK细胞活性也在先兆子痫妇女IUGR中增强。内皮细胞（EC）在重度先兆子痫患者血清中孵育过夜。当在含有患者血清的培养基中培养时，EC上ICAM-1的表达明显增强。这些结果表明，有一个因素，增强ICAM-1的表达， ...更多信息 2.自然流产与细胞粘附分子研究注射抗ICAM-1抗体和抗LFA-1抗体的动物移植心脏存活，自然流产被认为是母体免疫系统对半同种异体移植心脏的排斥反应。我们研究了在小鼠自发流产模型CBAXDBA/2交配中注射抗粘附分子抗体预防胎儿吸收的功效。将与DBA/2雄性小鼠交配的CBA/J雌性小鼠从妊娠第1天至第5天腹腔注射抗ICAM-1抗体和抗LFA-1抗体5天。在妊娠第13天，处死妊娠小鼠，计数存活和再吸收胚胎。虽然抗体注射组和对照组（盐水注射）的着床位点数之间没有显著差异，但抗体处理组的活胚胎数显著增加。这些结果表明，自发性胎儿吸收可以通过控制细胞粘附分子介导的细胞相互作用来预防。少