Pregnancy and cell adhesion molecules ; Role of cell adhesion molecules in the pathogenesis of spontaneous abortion and preeclampsia

1.子痫前期和细胞粘附分子血管内皮细胞（EC）损伤被认为在子痫前期的发病机制中起核心作用。自然杀伤细胞可能是内皮细胞以及中性粒细胞、巨噬细胞和细胞毒性t淋巴细胞的可能效应物之一。有报道称细胞粘附分子ICAM-1和IFA-1可能参与NK细胞对ECs的粘附。这些证据使我们能够研究NK细胞和血管内皮细胞在子痫前期发病机制中的相互作用。NK细胞活性在严重蛋白尿的子痫前期妇女中被发现显著增强。NK细胞活性在IUGR的子痫前期妇女中也增强。内皮细胞（ECs）在严重子痫前期患者血清中孵育过夜。在含有患者血清的培养基中培养时，ECs上ICAM-1的表达明显增强。这些结果提示，在ECs细胞表面存在一种能增强ICAM-…More 1表达的因子，NK细胞与ECs的相互作用可能参与了子痫前期的发病机制。自然流产和细胞粘附分子注射抗icam -1抗体和抗lfa -1抗体后移植心脏存活，自然流产被认为是母体免疫系统对半同种异体移植物的排斥反应。在小鼠自然流产模型CBAXDBA/2交配中，我们观察了注射抗粘附分子抗体对胎儿再吸收的影响。CBA/J雌性小鼠与DBA/2雄性小鼠交配后，从妊娠第1天至第5天，腹腔注射抗icam -1抗体和抗lfa -1抗体5 d。妊娠第13天处死孕鼠，计数活胚和再吸收胚。虽然注射抗体组的着床部位数与对照组（注射生理盐水组）无显著差异，但注射抗体组的活胚数显著增加。这些结果表明，可以通过控制细胞粘附分子介导的细胞相互作用来阻止胎儿自发吸收。少

1.Preeclampsia and Cell Adhesion MoleculesVascular endothelial cell (EC) injury is thought to play a central role in the pathogenesis of preeclampsia. Natural killer cells might be one of the possible effectors targeting endothelial cells as well as neutrophils, macrophages and cytotoxic T-Iymphocytes. It has been reported that cell adhesion molecule, ICAM-1 and IFA-1 could be involved in NK cell adhesion to ECs. These evidences allowed us to investigate the interaction between NK cells and vascular ECs in the pathogenesis of pre-eclampsia. NK cell activity in preeclamptic women with severe proteinuria found to be significantly augmented. NK cell activity was also augmented in pre-eclamptic women with IUGR.Endothelial cells (ECs) were incubated overnight in the presence of patient sera with severe preeclampsia. ICAM-1 expression on ECs was clearly enhanced when cultured in medium containing patient sera. These results suggest that there is a factor which enhance the expression of ICAM- … More 1 on the cell surface of ECs and that the interaction between NK cells and ECs might be involved in the pathogenesis of pre-eclampsia.2.Spontaneous abortion and Cell Adhesion MoleculesTransplanted heart graft survives in the animals injected with anti-ICAM-1 antibody and anti-LFA-I antibody The spontaneous abortion is regarded as a rejection of semiallograft by maternal immune system. We investigated an efficacy of the injection of anti-adhesion molecule antibody to prevent fetal resorptions in the murine spontaneous miscarriage model, CBAXDBA/2 matings. CBA/J female mice mated with DBA/2 male mice were injected intraperitoneally with anti-ICAM-1 antibody and anti-LFA-I antibody for 5 days from day I to 5 of gestation. On day 13 of gestation, pregnant mice were sacrificed and viable and resorbing embryos were counted. Although there was not a significant difference between the number of implantation sites for antibody injected group and that for control (saline injected) group, the number of living embryo was significantly increased in antibody treated group. These results suggest that spontaneous fetal resorption can be prevented by controlling cellular interaction mediated by cell adhesion molecules. Less