Mechanism of Bone Destruction Due to Middle Ear Cholesteatoma : Experimental Study

中耳胆脂瘤引起的骨破坏机制：实验研究

• 批准号: 07671860
• 负责人: OHSAKI Katsuichiro
• 金额: $ 1.47万
• 依托单位: University Hospital. School of Medicine. The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671860/
• 关键词: middle ear cholesteatoma; bone destruction mechanism; laser-Raman Spectroscopy; hydroxyapatite; Apaceram; demineralization; remineralization; molecular level; middle ear cholesteatoma; bone destruction mechanign; Apaceram; remineralization; la

Details of the mechanism of bone destruction due to middle ear cholesteatoma still remain obscure. In this study, we used laser-Raman spectroscopy to study tissue at the molecular level and based on our results, proposed a hypothesis to explain the process of bone destruction occurring in chronic otitis media associated with cholesteatoma. In this destruction, remineralization may take place in combination with demineralization, and hydroxyapatite [Ca_<10>(PO_4)_6(OH)_2 : HOAP] or its analogues may be deposited on the destroyed bone surface under mildly acidic conditions [Otologia (Fukuoka) 1983, Am J Otolaryngol 1988].To obtain basic data to verify this hypothesis, model simulation in vivo and in vitro were performed. In order to simplify the model system, we paid attention to HOAP,which largely consists of inorganic constituents of mature bone and regarded HOAP as typical specimen of the mineral component of the bone. As the experimental specimen, synthetic auditory ossicle (Apaceram … More ^<<encircledR>>) made from HOAP was employed. In this study, we present model experiment for the de- and remineralization processes at molecular level using a laser-Raman spectrometer.In vivo experiment, the Apaceram discs were implanted subcutaneously into the interscapular region of rats. Untreated Apaceram was observed as a control. In vitro experiment, we carried out consecutive measurement of the ions released in saline solution in which cylinders of Apaceram had been immersed. Results are as follows : 1. Apaceram surface : not only PO_4^<3-> of HOAP but also very small amounts of phosphate were considered to be present. (2) dissolution of ions from Apaceram surface had been increased with time in vitro experiment and demineralization of Apaceram surface had been progressed at 6 months after implantation and in contrast, precipitation of phosphate other than HOAP had occurred at 10 months after implantation. 2. Tissue in contact with Apaceram surface : the more immatured substances like bone at 3 months and osseou substances at 6 or 10 months were observed on the subcutaneous tissue. These data mean that deposition of HOAP progressed with time after implantation.As a result of model simulation in rats de- and remineralization were demonstrated on the Apaceram surface by laser-Raman spectrometry.In conclusion, our proporsed hypothesis on the bone destruction mechanism due to chronic otitis media associated with cholesteatoma will be supported by the experimental data analyzed at molecular level, considering HOAP which is the main inorganic constituent of mature bone. Less

中耳乳突瘤造成骨质破坏的机制仍不清楚。在这项研究中，我们使用激光拉曼光谱在分子水平上研究组织，并根据我们的结果，提出了一个假设来解释与中耳炎相关的慢性中耳炎中发生的骨破坏的过程。在这种破坏过程中，骨的矿化可能与脱矿同时发生，在弱酸性条件下，羟基磷灰石[Ca_<10>（PO_4）_6（OH）_2：HOAP]或其类似物可能沉积在破坏的骨表面[Otologia（Fukuoka）1983，Am J Otolaryngol 1988]。为了简化模型系统，我们注意到HOAP，它主要由成熟骨的无机成分组成，并将HOAP视为骨矿物成分的典型标本。以人工合成听小骨（Apaceram ...更多信息 ^&lt;<encircledR>&gt;）。在本研究中，我们提出了一个模型实验，在分子水平上使用激光拉曼光谱仪。在体内实验中，Apaceram光盘植入大鼠肩胛间皮下区域。观察未处理的Apaceram作为对照。在体外实验中，我们进行了连续的离子释放在盐溶液中，其中的圆柱体的Apaceram已被浸泡。研究结果如下：1. Apaceram表面：不仅存在HOAP的PO_4 ~+<3->，而且存在极少量的磷酸盐。(2)在体外实验中，离子从Apaceram表面的溶解随着时间的推移而增加，并且在植入后6个月，Apaceram表面的脱矿作用已经进行，相反，在植入后10个月，除了HOAP之外，已经发生磷酸盐沉淀。2.与Apaceram表面接触的组织：在皮下组织上观察到较不成熟的物质，如3个月时的骨和6或10个月时的骨质。这些数据意味着HOAP的沉积随着植入后时间的推移而进行。作为大鼠模型模拟的结果，通过激光拉曼光谱法证实了Apaceram表面的去矿化和再矿化。总之，我们关于慢性中耳炎与中耳炎相关的骨破坏机制的比例假设将得到分子水平上分析的实验数据的支持。考虑到HOAP是成熟骨的主要无机成分。少

项目成果

Katsuichiro Ohsaki: "Surface structure of synthetic anditory ossicle(Apaceram^<【encircledR】>)in planted in the suburtaneous tissue of rats" Proccedings of the ^+XVI World Congress of Otherhine laryngology Head and Neck Surgery. (in press). (1997)

Katsuichiro Ohsaki：“植入大鼠皮下组织的合成听小骨（Apaceram^<【encircledR】>）的表面结构”^+XVI 世界其他喉头颈外科大会记录（1997 年）。 ）

Katsuichiro Ohsaki et al.: "Demostrations of de- and remineralization mechanism as revealed in synthetic auditory ossicle (Apaceram<@D1(]SY.encircled.C[)@>D1) of rats by Laser-Raman Spectrometory" Cellular and Molecular Biology. 41(8). 1155-1167 (1997)

Katsuichiro Ohsaki 等人：“通过激光拉曼光谱法演示大鼠合成听小骨 (Apaceram<@D1(]SY.encircled.C[)@>D1) 的脱矿和再矿化机制”细胞和分子生物学

Katsuichiro Ohsaki et al.: "Surface structure of synthetic auditory ossicle (Apaceram<@D1(]SY.encircled.C[)@>D1) implanted in the subcutaneous tissue of rats" Proceedings of the "XVI World Congress of Otorhinolaryngology Head and Neck Surgery, Monduzzi Ed

Katsuichiro Ohsaki 等：“植入大鼠皮下组织的合成听小骨 (Apaceram<@D1(]SY.encircled.C[)@>D1) 的表面结构”《第十六届世界耳鼻咽喉科学大会论文集》

Katsuichiro Ohsaki: "Demostrations of de-and remineralization mechanism as revealed in synthetic auditory ossicle(Apaceram^<【encircledR】>)of rats by Laser-Raman Spectrometory" Cellular and Molecular Biology. 41. 1155-1167 (1995)

Katsuichiro Ohsaki：“通过激光拉曼光谱揭示大鼠合成听小骨（Apaceram^<【circledR】>）的去矿化机制”细胞和分子生物学 41. 1155-1167（1995）。

Katsuichiro Ohsaki: "Surface Structure of a Syhtletic andltory ossicle(Apaceram【encircledR】)implanted in the saburtaneaus Tissue of rats" Proceedings of the XV1 world Congress of Otorhino-laryngology Head and Neck Surgery. (in press). 1997

Katsuichiro Ohsaki：“植入大鼠 saburtaneaus 组织中的 Syhtletic 听小骨（Apaceram【circledR】）的表面结构”，1997 年第 XV1 届世界耳鼻喉头颈外科大会论文集。