Histological Research of Soft Tissue and Auditory Bulla Bone, and Microstructural Analyses of synthetic Auditory Ossicle in Rats

大鼠软组织和听大骨的组织学研究以及合成听小骨的显微结构分析

• 批准号: 10671594
• 负责人: OHSAKI Katsuichiro
• 金额: $ 1.41万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671594/
• 关键词: synthetic auditory ossicle; subcutaneous tissue; Raman spectra; Histology; auditory bulla; mineralization; microscopies; rats; hydroxyapatite; histomorphometry; auditory bulla bone; laser-Roman Spectrometry; alumium Oxide; long-term reaction

The synthetic auditory ossicle alumina oxide (AlィイD22ィエD2OィイD23ィエD2, Bioceram) and hydroxyapatite [HA : CaィイD210ィエD2(POィイD24ィエD2)ィイD26ィエD2(OH)ィイD22ィエD2] are currently used widely in reconstructive middle ear surgery.To evaluate biocompatibility to tissue in long-term implantation, Bioceram disks were implanted subcutaneously in rats for 20 months. Light microscopy of H&E specimens differentiated cell types. Histological response included some macrophages and lymphocytes at six month which gradually disappeared by 16 months. The findings indicate that, from the viewpoint of long-term tissue response, Bioceram is a satisfactory biocompatlble material for reconstructive surgery.To investigate the long-term microstructure of Apaceram (Ap), a synthetic auditory ossicle composed of dense HA, thin Ap disks were implanted subcutaneaously in rats for 20 months. Implanted surfaces observed using stereoscopic microscopy and scanning elecron microscopy indicated progressive degradation on disk sur … More faces of the implants and later, progressive redeposition. Raman spectra analysis demonstrated that demineralization occurred on disk surfaces of implants, followed by progressive remineralization. Our histological study showed that, with increase in time after implantation, fibrous capsule thickness (FCT) increased and fibrous capsules surrounding Ap disks became denser. Increase of FCT and high density of collagen fibers could have restricted diffusion of ions passing through dense fibrous capsules located in the interface regions (body fluid phase) between Ap and capsules. The balance between demineralization and remineralization would then have been maintained under a restricted condition for ion diffusion. These histopathological findings supported our earlier hypothesis of Apaceram model (Cell mol Biol, 1995), that remineratization is accompanied by environmental change in the soft tissue. Apaceram seems useful for reconstructive surgery, since remineralization allows implanted Apaceram to maintain almost its original size in vivo.To investigate the bicompatibility of synthetic auditory ossicle (Ap) to host bone, small Ap disks contacting on the auditory bulla bone in rats were implanted for 270 days. The experiment showed : 1) no evidence of inflammatory reaction caused by implants from 90 days after implantation, 2) no evidence of osteolysis caused by implants, and 3) direct contact of bone to implants on the bone-disk interfaces at 180 and 270 days after implantation. The findings suggest that Ap has a high degree of implant biacompatibility, making it a satisfactory substitute biomaterial for otological reconstructive surgeries. Less

人工合成听骨CaィイD210ィエD2(POィイD24ィエD2)ィイD26ィエD2(OH)ィイD22ィエD2](AlィイD22ィエD2OィイD23ィエD2，Bioceram)和羟基磷灰石[HA：Bioceram]是目前广泛应用于中耳重建手术的材料。光镜观察H&E标本分化的细胞类型。组织学反应在6个月时出现一些巨噬细胞和淋巴细胞，16个月后逐渐消失。结果表明，从长期组织反应的角度来看，Bioceram是一种良好的重建手术生物相容性材料。为了研究APaceram(AP)的长期微结构，将由致密的HA、AP薄片组成的人工听骨植入大鼠皮下20个月。立体显微镜和扫描电子显微镜观察到的植入表面显示…表面有进行性降解。植入物的更多面孔和后来的渐进性再沉积。拉曼光谱分析表明，种植体盘状表面发生了脱矿，随后逐渐发生再矿化。我们的组织学研究表明，随着植入后时间的延长，纤维包膜厚度(FCT)增加，AP盘周围的纤维包膜变得更加致密。FCT的增加和胶原纤维的高密度可能限制了离子通过位于AP和胶囊之间的界面区域(体液相)的致密纤维胶囊的扩散。在离子扩散受到限制的情况下，脱矿和再矿化之间的平衡将得到维持。这些组织病理学发现支持我们早期的Apaceram模型(Cell MolBiol，1995)的假说，即软组织中的再矿化伴随着环境的变化。Apaceram似乎对重建手术有用，因为再矿化使植入的Apaceram在活体中几乎保持其原始大小。为了研究合成听骨(AP)与宿主骨的双相容，将接触到听泡骨的AP小圆盘植入大鼠听泡骨270天。实验结果表明：1)种植后90天未见种植体引起炎症反应；2)种植后未见骨溶解；3)种植后180天和270天种植体与骨盘界面有直接接触。结果表明，AP具有高度的种植体生物相容性，是一种理想的耳科重建手术替代材料。较少

项目成果

Katsuichiro Ohsaki et al.: "Subcutaneous inflammatory reaction to a synthetic auditory ossicle (Bioceram【encircledR】) in rats"Acta Oto-Laryngologica (Stockholm). 119(1). 83-88 (1999)

Katsuichiro Ohsaki 等人：“大鼠合成听小骨（Bioceram【圈出的R】）的皮下炎症反应”Acta Oto-Laryngologica (斯德哥尔摩) 83-88 (1999)。

Katsuichiro Ohaski et al.: "Thickness of fibrous capsule after implantation of hydroxyapatite in subcutaneous tissue in rats"Journal of Biomedical Materials Research. 45(4). 322-326 (1999)

Katsuichiro Ohaski 等人：“将羟基磷灰石植入大鼠皮下组织后纤维囊的厚度”生物医学材料研究杂志。

Katsuichiro Ohasaki et al.: "Long-term microstractural analyses of hydroxyapatite implanted in rats using laser-Raman spectrometry and scanning electron microscopy"Cellular and Molecular Biology. 45(6). 793-803 (1999)

Katsuichiro Ohasaki 等人：“使用激光拉曼光谱和扫描电子显微镜对植入大鼠体内的羟基磷灰石进行长期微结构分析”细胞和分子生物学。

Katsuichiro Ohsaki: "Histological reaction of auditory bulla bone to synthetic auditory ossicle (Apaceram【○!R】) in rats"The Journal of Medical Investigation. 47(1,2). in press (2000)

Katsuichiro Ohsaki：“大鼠听大骨对合成听小骨（Apaceram【○！R】）的组织学反应”医学调查杂志47（1,2）出版。

Katuichiro Ohsaki: "Subcutaneous Inflammatory Reaction to a Synthetic Auditory Ossicle(Bioceram)in Rats" Acta Otolarygologica(Stockholn). 119(1). 83-88 (1999)

Katuichiro Ohsaki：“大鼠合成听小骨（Bioceram）的皮下炎症反应”Acta Otolarygologica（斯德哥尔恩）。