Histological Research of Soft Tissue and Auditory Bulla Bone, and Microstructural Analyses of synthetic Auditory Ossicle in Rats

The synthetic auditory ossicle alumina oxide （Al-D22-D2O-D23-D2， Bioceram） and hydroxyapatite [HA ： Ca-D210-D2（PO-D24-D2）-D26-D2（OH）-D2] are currently used widely in reconstructive middle ear surgery。To evaluate biocompatile to tissue in long-term implantation， Bioceram disks were implanted subcutaneously in rats for 20 months。H&E特异性细胞类型的光显微镜。历史性反应包括6个月内16个月内的一些宏观细胞和淋巴细胞。根据这一发现，从长期组织反应的角度来看，生物色素是一种用于重建手术的饱和生物相容材料。为了调查Apaceram （Ap）的长期微结构，一种由密集型HA组成的合成auditor osicle，薄App磁盘被植入了亚切可移植到大鼠20个月。植入式表面观察使用立体镜显微镜和扫描电子显微镜显微镜指示性的渐进式退化在磁盘上 ... More 植入物和以后的阶段，渐进式还原。拉曼特别分析演示了在植入物的磁盘表面上出现的去量化，并由渐进式再量化跟踪。我们的历史学研究显示，随着植入物的增加，纤维胶囊厚度（FCT）增加，纤维胶囊周围的纤维胶囊变成了皮肤干燥器。FCT的增加和聚合纤维的高密度可能具有限制性的扩散，通过密集纤维胶囊定位在接口区域（体流体阶段）之间的阿普和胶囊。在电离化和还原化之间的平衡，然后就可以在离子扩散的受限制条件下保持。这些历史学发现支持我们早期Apaceram模型的催眠（Cell mol Biol， 1995年），即还原是由软组织中的环境变化结合的。Apaceram似乎对再构建性手术有用，因为重新简化允许植入Apaceram以维持其原始尺寸在体内，以调查合成auditorosicle（App）的双耐受性与宿主骨骼和小App磁盘在大鼠植入270天内接触。该实验展示了：1）植入后90天内没有植入物引起的炎症基质反应的证据; 2）植入后没有骨质溶解引起的证据;以及3）植入后180天和270天内骨骼与植入物上的骨骼直接接触。研究结果建议App具有较高程度的植入物生物相容性，并使其成为专业重建手术的一种饱和因子替代生物材料。Less（低）

The synthetic auditory ossicle alumina oxide (AlィイD22ィエD2OィイD23ィエD2, Bioceram) and hydroxyapatite [HA : CaィイD210ィエD2(POィイD24ィエD2)ィイD26ィエD2(OH)ィイD22ィエD2] are currently used widely in reconstructive middle ear surgery.To evaluate biocompatibility to tissue in long-term implantation, Bioceram disks were implanted subcutaneously in rats for 20 months. Light microscopy of H&E specimens differentiated cell types. Histological response included some macrophages and lymphocytes at six month which gradually disappeared by 16 months. The findings indicate that, from the viewpoint of long-term tissue response, Bioceram is a satisfactory biocompatlble material for reconstructive surgery.To investigate the long-term microstructure of Apaceram (Ap), a synthetic auditory ossicle composed of dense HA, thin Ap disks were implanted subcutaneaously in rats for 20 months. Implanted surfaces observed using stereoscopic microscopy and scanning elecron microscopy indicated progressive degradation on disk sur … More faces of the implants and later, progressive redeposition. Raman spectra analysis demonstrated that demineralization occurred on disk surfaces of implants, followed by progressive remineralization. Our histological study showed that, with increase in time after implantation, fibrous capsule thickness (FCT) increased and fibrous capsules surrounding Ap disks became denser. Increase of FCT and high density of collagen fibers could have restricted diffusion of ions passing through dense fibrous capsules located in the interface regions (body fluid phase) between Ap and capsules. The balance between demineralization and remineralization would then have been maintained under a restricted condition for ion diffusion. These histopathological findings supported our earlier hypothesis of Apaceram model (Cell mol Biol, 1995), that remineratization is accompanied by environmental change in the soft tissue. Apaceram seems useful for reconstructive surgery, since remineralization allows implanted Apaceram to maintain almost its original size in vivo.To investigate the bicompatibility of synthetic auditory ossicle (Ap) to host bone, small Ap disks contacting on the auditory bulla bone in rats were implanted for 270 days. The experiment showed : 1) no evidence of inflammatory reaction caused by implants from 90 days after implantation, 2) no evidence of osteolysis caused by implants, and 3) direct contact of bone to implants on the bone-disk interfaces at 180 and 270 days after implantation. The findings suggest that Ap has a high degree of implant biacompatibility, making it a satisfactory substitute biomaterial for otological reconstructive surgeries. Less