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The Etiology of Endolymphatic Hydrops-Role of Spiral Ligament and Endolymphatic Sac-

内淋巴积水的病因学-螺旋韧带和内淋巴囊的作用-

基本信息

批准号：
07671868
负责人：
ICHIMIYA Issei
金额：
$ 1.47万
依托单位：
Oita Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671868/
关键词：
endolymphatic hydrops spiral ligament endolymphatic sac keyhole limpet hemocyanin immunohistochemistry connexin 26 fibrocytes extracellular matrix vimentin S-100 connexin26 proteoglycan collagen ラセン靱帯 Na,K-ATPase gap junction

项目摘要

1)Labyrinthitis was induced by inoculation of keyhole limpet hemocyanin into the scala tympani of systemically sensitized guinea pigs, and inner ears were immunostained. Spiral ligament, spiral limbus, and blood vessels including the spiral modiolar vein were diffusely positive for IgG and albumin. Immunoreactivity for connexin 26 and Na-K-ATPase was decreased in the fibrocytes of the spiral ligament, however, endolymphatic hydrops was not induced.2)Changes in cochleas with experimental otitis media were investigated in mice. Otitis media was induced in all of the animals after the inoculation of Streptococcus pneumoniae into the middle ear cavity, and some of them showed inflammatory cells in the cochlea. Although other changes, including endolymphatic hydrops, were not obvious, cochleas showed positive immunostaining for fibrinogen. Immunostaining for connexin 26 was slightly decreased in the spiral ligament, accompanied by fibrinogen staining.3)Endolymphatic sacs of the guinea pigs … More were ablated to induce endolymphatic hydrops and processed for immunohistochemistry. Staining of type I fibrocytes in the spiral ligament, which are positive for vimentin and connexin 26, was decreased. However, immunostaining for extracellular matrix in the spiral ligament, which are positive for chondroitin 4-sulfate proteogly can and type II collagen, showed no changes.Because endolymphatic hydrops is not always obseved with the changes in the fibrocytes of the spiral ligament, it is suggested that the spiral ligament is not responsible for the production of endolymphatic hydrops. However, spiral ligament appears to be responsible for the cochlear malfunction, and immunostaining for fibrinogen or other blood constituents suggests that the blood-labyrinth barrier could disrupt in the spiral ligament by labyrinthitis or otitis media. The constant staining for the extracellular matrix with the experimental endolymphatic hydrops raises the possibility that changes in the spiral ligament has been underestimated by routine otopathologic observations. Less

1)通过将匙孔血蓝蛋白接种到全身致敏豚鼠的鼓阶中来诱导实验性唇炎，并对内耳进行免疫染色。螺旋韧带、螺旋利姆布斯和包括螺旋蜗轴静脉在内的血管呈IgG和白蛋白弥漫阳性。螺旋韧带纤维细胞中连接蛋白26和钠钾ATP酶的免疫反应性降低，但未诱导内淋巴积水。2）观察实验性中耳炎小鼠耳蜗的变化。中耳腔接种肺炎链球菌后，所有动物均出现中耳炎，部分动物耳蜗内可见炎性细胞。虽然其他变化，包括内淋巴积水，是不明显的，耳蜗纤维蛋白原免疫染色呈阳性。连接蛋白26在螺旋韧带中的免疫染色略有减少，并伴有纤维蛋白原染色。3）豚鼠内淋巴囊 ...更多信息消融以诱导内淋巴积水并进行免疫组织化学处理。螺旋韧带中波形蛋白和连接蛋白26阳性的I型纤维细胞染色减少。但螺旋韧带内细胞外基质免疫组化染色显示4-硫酸软骨素蛋白聚糖和II型胶原蛋白阳性，未见明显变化，由于内淋巴积水并不总是伴随着螺旋韧带纤维细胞的变化，因此，螺旋韧带与内淋巴积水的产生无关。然而，螺旋韧带似乎是耳蜗功能障碍的原因，纤维蛋白原或其他血液成分的免疫染色表明，血液迷路屏障可能会因中耳炎或中耳炎而破坏螺旋韧带。实验性内淋巴积水的细胞外基质持续染色，提示常规耳病理学观察低估了螺旋韧带的变化。少