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Ultrastructural studies of the multinucleation of osteoclasts ; the role and function of mononuclear osteoclasts.

破骨细胞多核的超微结构研究；

基本信息

批准号：
07671959
负责人：
DOMON Takanori
金额：
$ 1.54万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671959/
关键词：
odontoclast osteoclast distribution of number of nuclei mononuclear cell multinucleation 連続切片単核

项目摘要

Osteoclasts and odontoclasts have been considered multinucleated giant cells which resorb hard tissue by ruffled borders. Recently, the authors reported the presence of a nomonuclear osteoclast and odontoclast with a ruffled border. However, the relative frequency of such cells and the distribution of the number of nuclei including mononuclear cells in them have not been elucidated.Six human deciduous teeth were used in this study. After fixation and decalcification, tartrate-resistant acid phosphatase (TRAP) activity was detected with the azo dye method, and then TRAP-positive cells were observed on resorbing areas of teeth by light microscopy. The cells for investigation were serially sectioned by semithin sections to observe the presence of resorptive lacuna and the number of nuclei.The TRAP activity was detected in both multinucleated and mononuclear odontoclasts from serial semithin sections, and 242 TRAP-positive cells which formed lacunae on dentin were investigated to determinie the frequency distribution of the number of nuclei. The mean number of nuclei per cell was 5.3, and median was 4. Only 2.9 per cent of odontoclasts were mononucleus and 93.8 per cent had 10 or fewer nuclei.The majority of odontoclasts forming lacunae on the dentin were cells with 10 or ferwer nuclei, and mononuclear odontoclasts parcipitated in human deciduous tooth resorption together with multinucleated ones.

破骨细胞和破牙本质细胞被认为是多核巨细胞，它们通过皱褶的边界吸收硬组织。最近，作者报道了存在一个nomandescent破骨细胞和破牙细胞与皱褶的边界。然而，这种细胞的相对频率和核的数量分布，包括单核细胞在其中还没有阐明。固定脱钙后，用偶氮染料法检测抗酒石酸酸性磷酸酶（TRAP）活性，光镜下观察牙齿吸收区TRAP阳性细胞。用半薄切片法连续观察牙本质细胞吸收陷窝的存在和细胞核的数目，并对242个形成陷窝的破牙本质细胞进行TRAP活性检测，以确定细胞核数目的频率分布。每个细胞的平均细胞核数为5.3，中位数为4。破牙本质细胞中只有2.9%为单核细胞，93.8%为10个或10个以下的细胞，在牙本质上形成陷窝的破牙本质细胞以10个或更多个核的细胞为主，单核破牙本质细胞与多核破牙本质细胞一起出现在人乳牙吸收过程中。