Analysis of Cell Adhesion Molecules and Stromal Factors Associated with Infiltration and Metastasis in Oral Squamous Cell Carcinoma

1.应用免疫组织化学方法检测nm23-H1蛋白在腺样囊性癌(ACC)和口腔鳞癌(OSCC)中的表达，并与其临床病理特征进行相关性分析。在ACC中，半数以上的病例nm23-H1表达降低，且多见于有转移和预后不良的病例。此外，与原发肿瘤相比，转移性肿瘤的免疫反应性降低。这些结果提示nm23-H1可能与口腔鳞癌的转移有关。在口腔鳞癌中，虽然约有一半的病例表达降低，但转移的频率与nm23-H1.2的染色程度无关。CD44v6在细胞膜上的表达已经减少，在发育不良的上皮细胞中呈异质性。CD44v9在口腔鳞癌中的表达明显降低，其表达与口腔鳞癌的淋巴结转移和预后密切相关。CD44表达的改变可能在肿瘤的转移中起一定作用。3.P53和bc1-2蛋白的积聚与肿瘤细胞对放射治疗的抵抗有关。肿瘤间质中的成纤维细胞、巨噬细胞和血管内皮细胞表达转化生长因子β，可作为自分泌或旁分泌系统刺激内皮细胞的增殖。5.肿瘤细胞表达纤溶酶原激活物。这一发现表明，癌细胞本身对基质的破坏有积极的帮助。

1.The expression of nm23-H1 protein was evaluated immunohistochemically in adenoid cystic carcinoma (ACC) and oral squamous cell carcinoma (OSCC) and correlated with their clinicopathologic features. In ACC,more than half cases showed the reduction of nm23-H1 expression and the reduction was more commonly observed in the cases with metastasis and unfavorable prognosis. Furthermore, metastatic tumors showed decreased immunoreactivity compared with their primary tumors. These results may suggest that nm23-H1 has an implication for metastasis of ACC.In OSCC,although about half cases showed the reduced expression, there was no relationship between the frequency of metastasis and the stainability of nm23-H1.2. The expression of CD44v6 on the cytomembrane already decreased and became heterogenous in the dysplastic epithelium. The reduced expression of CD44v9 was frequently observed in OSCC and its was significantly related to lymph node metastasis and worse prognosis. The alteration of CD44 expression may have some role in metastasis.3.The accumulation of p53 and bc1-2 proteins was related to the resistance of cancer cells to radiation therapy. Apoptosis of the cancer cells induced by irradiation may partly be inhibited by these proteins in OSCC.4.TGF-beta, one of the vascular growth factors, was expressed in fibroblasts, macrophases and vascular endothelial cells in the cancer stroma and may act as autocrine or paracrine system to stimulate the proliferation of endothelial cells.5.Plasminogen activator was expressed in cancer cells as well as in macrophases. This finding suggests that the cancer cell itself give positive aid to destruction of the stroma.