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Analysis of Cell Adhesion Molecules and Stromal Factors Associated with Infiltration and Metastasis in Oral Squamous Cell Carcinoma

口腔鳞状细胞癌浸润和转移相关的细胞粘附分子和基质因子分析

基本信息

批准号：
07671972
负责人：
OGAWA Ikuko
金额：
$ 1.47万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

1.The expression of nm23-H1 protein was evaluated immunohistochemically in adenoid cystic carcinoma (ACC) and oral squamous cell carcinoma (OSCC) and correlated with their clinicopathologic features. In ACC,more than half cases showed the reduction of nm23-H1 expression and the reduction was more commonly observed in the cases with metastasis and unfavorable prognosis. Furthermore, metastatic tumors showed decreased immunoreactivity compared with their primary tumors. These results may suggest that nm23-H1 has an implication for metastasis of ACC.In OSCC,although about half cases showed the reduced expression, there was no relationship between the frequency of metastasis and the stainability of nm23-H1.2. The expression of CD44v6 on the cytomembrane already decreased and became heterogenous in the dysplastic epithelium. The reduced expression of CD44v9 was frequently observed in OSCC and its was significantly related to lymph node metastasis and worse prognosis. The alteration of CD44 expression may have some role in metastasis.3.The accumulation of p53 and bc1-2 proteins was related to the resistance of cancer cells to radiation therapy. Apoptosis of the cancer cells induced by irradiation may partly be inhibited by these proteins in OSCC.4.TGF-beta, one of the vascular growth factors, was expressed in fibroblasts, macrophases and vascular endothelial cells in the cancer stroma and may act as autocrine or paracrine system to stimulate the proliferation of endothelial cells.5.Plasminogen activator was expressed in cancer cells as well as in macrophases. This finding suggests that the cancer cell itself give positive aid to destruction of the stroma.

1.应用免疫组织化学方法检测nm23-H1蛋白在腺样囊性癌（ACC）和口腔鳞状细胞癌（OSCC）中的表达，并分析其与临床病理特征的相关性。在ACC中，超过一半的病例出现nm23-H1表达降低，且这种降低多见于转移和预后不良的病例。此外，与原发肿瘤相比，转移性肿瘤的免疫反应性降低。这些结果可能提示nm23-H1与ACC的转移有关。在OSCC中，虽然约有一半的病例表达降低，但转移频率与nm23-H1.2的染色性无关。CD44v6在细胞膜上的表达已经减少，在发育不良的上皮中呈异质表达。CD44v9在OSCC中表达降低较多，与淋巴结转移及预后不良有显著关系。CD44表达的改变可能在转移中起一定作用。p53和bc1-2蛋白的积累与癌细胞对放射治疗的抵抗有关。在OSCC.4中，这些蛋白可能在一定程度上抑制辐照诱导的癌细胞凋亡。tgf - β是血管生长因子之一，在癌间质成纤维细胞、巨相细胞和血管内皮细胞中表达，可作为自分泌或旁分泌系统刺激内皮细胞增殖。纤溶酶原激活物在肿瘤细胞及巨期均有表达。这一发现表明癌细胞本身对基质的破坏起到了积极的作用。