DEVELOPMENT OF A NEW ENAMEL BIOPSY METHOD BY ESCA

ESCA 开发新的牙釉质活检方法

• 批准号: 07672083
• 负责人: KAWAKAMI Tomofumi
• 金额: $ 1.15万
• 依托单位: HEALTH SCIENCES UNIVERSITY OF HOKKAIDO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672083/
• 关键词: ESCA; ENAMEL BIOPSY; HYDROXYAPATITE; ESCA; ヒト歯質

Electron Spectroscopy of Chemical Analysis (ESCA) was used as a new method for analysis of tooth hydroxyapatite {Ca_<10>(PO_4)_6(OH)_2}. By quantitative analysis of Ca, P and O of human sound enamel and dentin, I recognized the differences of the composition and structure of hydroxyapatite crystal among teeth as well as between enamel and dentin. In this study, I attempted to develop a new enamel biopsy method using ESCA to elucidate the tooth condition which determines caries susceptibility.The extremely small amount of samples (0.1mg) were obtained by using the polishing disk and strips from the patients' teeth. The Ca/P ratio of the patients' enamel was consistent with that of enamel in the extracted teeth. However, the ratio of oxygen bound to phosphorous and bound to hydrogen of the patients' enamel, which is thought to be an important factor for determination of caries susceptibility of the patient's tooth, could not be obtained. I attributed this failure to the contamination of the tooth components such as Ca, P and O in the polishing disk and strips and the method of getting the samples. Then, I recognized the usefulness of titanium alloy as the material which does not contain the tooth components. Moreover, I improved the method to obtain the samples by providing the fine grooves on the strips.Following these results, I developed the method using titanium alloy as the base material of the strips to get the sample without the damage of tooth substrate. The some improvements concerned with the thickness of titanium alloy on the strips are necessary to establish the enamel biopsy method using ESCA.

化学分析电子能谱(ESCA)作为一种分析牙齿羟基磷灰石{Ca_<10>(PO_4)_6(OH)_2}的新方法。通过对人体健全牙釉质和牙本质中Ca、P、O的定量分析，认识到牙齿之间以及牙釉质和牙本质之间羟基磷灰石晶体的成分和结构的差异。在这项研究中，我尝试开发一种使用 ESCA 的新牙釉质活检方法，以阐明决定龋齿易感性的牙齿状况。通过使用抛光盘和牙条从患者牙齿中获取极少量的样本（0.1 毫克）。患者牙釉质的Ca/P比值与拔除牙齿的牙釉质一致。然而，患者牙釉质中与磷结合的氧和与氢结合的氧的比率无法获得，而该比率被认为是确定患者牙齿龋齿易感性的重要因素。我将此故障归因于抛光盘和抛光条中Ca、P、O等牙齿成分的污染以及取样方法。然后，我认识到钛合金作为不含牙齿成分的材料的有用性。此外，我改进了通过在条带上提供细槽来获取样本的方法。根据这些结果，我开发了使用钛合金作为条带基材的方法，以在不损坏牙齿基底的情况下获取样本。建立ESCA牙釉质活检方法需要对条带上钛合金厚度进行一些改进。

项目成果

川上 智史: "ESCAによるヒト歯質の分析(第1報)" 日本歯科保存学会雑誌. 38. 1205-1211 (1995)

Satoshi Kawakami：“使用 ESCA 分析人类牙齿结构（第 1 次报告）”日本保守牙科学会杂志 38. 1205-1211（1995 年）。

川上 智史: "ESCAによるヒト歯質の分析(第1報)" 日本歯科保存学会雑誌. 38(5). 1205-1211 (1995)

Satoshi Kawakami：“ESCA 对人类牙齿物质的分析（第 1 次报告）”日本保守牙科学会杂志 38（5）1205-1211（1995）。

Tomofumi KAWAKAMI,Hiroki OHNO and Koichi MATSUDA: "ESCA Study on Tooth Structures" Jpn.J.Conserv.Dent.38 (5). 1205-1211 (1995)

Tomofumi KAWAKAMI、Hiroki OHNO 和 Koichi MATSUDA：“ESCA 对牙齿结构的研究”Jpn.J.Conserv.Dent.38 (5)。