Mechanism of Recognition of Cells Denatured by Oxidative Stress

基于我们之前的发现，氧化损伤红细胞被抗3带糖蛋白自身抗体和巨噬细胞识别，我们阐明了氧化红细胞的识别机制，并研究了巨噬细胞对衰老和凋亡细胞的反应。1氧化细胞的识别机制1)氧化红细胞上抗3带自身抗体的配体是由氧化机制形成的3带聚集形成的聚n -乙酰乳胺基糖链簇。2) 3带糖链簇也被发现是巨噬细胞识别的决定因素。3)多种巨噬细胞膜蛋白具有聚n -乙酰乳糖氨基结合能力，可作为氧化红细胞的受体。纯化了其中一个分子量为50kD的蛋白，并对其n端10个氨基酸序列进行了分析。同源性分析表明，50kDa蛋白为新蛋白。体外培养的中性粒细胞作为衰老体细胞的模型，可以被巨噬细胞识别。虽然巨噬细胞识别凋亡细胞的决定因素尚不清楚，但某些药物诱导培养细胞凋亡可导致巨噬细胞识别。

Based on our previous findings that oxidatively damaged erythrocytes are recognized by anti-band 3 glycoprotein autoantibodies and macrophages, we have elucidated the mechanism of recognition of the oxidized erythrocytes, and examined response of macrophages against aged and apoptotic cells.I MECHANISM OF RECOGNITION OF OXIDIZED CELLS1) Ligands on oxidized erythrocytes for anti-band 3 autoantibodies were found to be clusters of poly-N-acetyllactosaminyl saccharide chains of band 3 formed by band 3 aggregation by oxidative mechanism.2) The clusters of the band 3 saccharide chains were also found to be the recognition determinants for macrophages.3) Multiple species of macrophage membrane proteins having poly-N-acetyllact osamine-binding ability were characterized as receptors for the oxidized erythrocytes. One of these proteins, with a molecular weight of 50kD,was purified and its N-terminal 10 amino acid sequence was elucidated. Result of homology search indicated that the 50kDa protein is a new protein.II MECHANISM OF RECOGNITION OF AGED CELLSNeutrophils incubated in vitro, a model of aged somatic cells, were found to be recognized by macrophages.III MECHANISM OF RECOGNITION OF APOPTOTIC CELLSInduction of apoptosis in cultured cells by some agents resulted in recognition by macrophages, although determinants on the apoptotic cells recognized by macrophages are unknown.