Mechanism of Recognition of Cells Denatured by Oxidative Stress

氧化应激变性细胞的识别机制

• 批准号: 07672390
• 负责人: BEPPU Masatoshi
• 金额: $ 1.6万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672390/
• 关键词: Oxidative stress; Host defense; macrophage lectin; Cell damage; Cell recognition; Polylactosamine; Aged erythrocyte; Apoptotic cell; マクロファージ; 老化細胞

Based on our previous findings that oxidatively damaged erythrocytes are recognized by anti-band 3 glycoprotein autoantibodies and macrophages, we have elucidated the mechanism of recognition of the oxidized erythrocytes, and examined response of macrophages against aged and apoptotic cells.I MECHANISM OF RECOGNITION OF OXIDIZED CELLS1) Ligands on oxidized erythrocytes for anti-band 3 autoantibodies were found to be clusters of poly-N-acetyllactosaminyl saccharide chains of band 3 formed by band 3 aggregation by oxidative mechanism.2) The clusters of the band 3 saccharide chains were also found to be the recognition determinants for macrophages.3) Multiple species of macrophage membrane proteins having poly-N-acetyllact osamine-binding ability were characterized as receptors for the oxidized erythrocytes. One of these proteins, with a molecular weight of 50kD,was purified and its N-terminal 10 amino acid sequence was elucidated. Result of homology search indicated that the 50kDa protein is a new protein.II MECHANISM OF RECOGNITION OF AGED CELLSNeutrophils incubated in vitro, a model of aged somatic cells, were found to be recognized by macrophages.III MECHANISM OF RECOGNITION OF APOPTOTIC CELLSInduction of apoptosis in cultured cells by some agents resulted in recognition by macrophages, although determinants on the apoptotic cells recognized by macrophages are unknown.

基于我们之前的发现，氧化损伤红细胞被抗3带糖蛋白自身抗体和巨噬细胞识别，我们阐明了氧化红细胞的识别机制，并研究了巨噬细胞对衰老和凋亡细胞的反应。1氧化细胞的识别机制1)氧化红细胞上抗3带自身抗体的配体是由氧化机制形成的3带聚集形成的聚n -乙酰乳胺基糖链簇。2) 3带糖链簇也被发现是巨噬细胞识别的决定因素。3)多种巨噬细胞膜蛋白具有聚n -乙酰乳糖氨基结合能力，可作为氧化红细胞的受体。纯化了其中一个分子量为50kD的蛋白，并对其n端10个氨基酸序列进行了分析。同源性分析表明，50kDa蛋白为新蛋白。体外培养的中性粒细胞作为衰老体细胞的模型，可以被巨噬细胞识别。虽然巨噬细胞识别凋亡细胞的决定因素尚不清楚，但某些药物诱导培养细胞凋亡可导致巨噬细胞识别。

项目成果

Masatoshi Beppu: "Poly-N-acetyllactosaminyl Saccharide Chains of Band 3 as Determinants for Anti-band 3 Autoantibody Binding to Senescent--" Cell.Mol.Biol.42. 1007-1024 (1996)

Masatoshi Beppu：“带 3 的聚 N-乙酰基乳糖胺基糖链作为抗带 3 自身抗体与衰老结合的决定因素 -”Cell.Mol.Biol.42。

Masatoshi Beppu: "Poly-N-acetyllactosaminyl:Saccharide Chains of Band 3 as determi-nants for Anti-band 3 Autoantibody Binding to Senescent and---" Cell.Mol.Biol.42(7). 1007-1024 (1996)

Masatoshi Beppu：“聚-N-乙酰基乳糖胺基：带 3 的糖链作为抗带 3 自身抗体与衰老结合的决定因素以及——”Cell.Mol.Biol.42(7)。

Masatoshi Beppu: "Recognition of Poly-N-acetyllactosaminyl Saccharide Chains on Iron-Oxidized Erythrocytes by Human Monocytic Leukemia Cell---" Biol.Pharm.Bull.19. 188-194 (1996)

Masatoshi Beppu：“人单核细胞白血病细胞对铁氧化红细胞上聚-N-乙酰基乳糖胺基糖链的识别---”Biol.Pharm.Bull.19。

Shigetoshi Eda: "Characterization of Lactoferrin-Binding Proteins of Human Macrophage Membrane : Multiple Species of Lactoferrin-Binding-" Biol.Pharm.Bull.20. 127-133 (1997)

Shigetoshi Eda：“人巨噬细胞膜乳铁蛋白结合蛋白的表征：乳铁蛋白结合的多种种类”Biol.Pharm.Bull.20。

Shigetoshi Eda et al.: "Characterization of lactoferrin-binding proteins of human macrophage membrane : Multiple species of lactoferrin-binding proteins with polylactosamine-binding ability." Biol.Pharm.Bull.20. 127-133 (1997)

Shigetoshi Eda 等人：“人巨噬细胞膜乳铁蛋白结合蛋白的表征：具有聚乳糖胺结合能力的多种乳铁蛋白结合蛋白。”