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Genetical and epidemiological analysis of enterococci resistant to vancomycin

万古霉素耐药肠球菌的遗传流行病学分析

基本信息

批准号：
07672492
负责人：
YAMANE Nobuhisa
金额：
$ 1.34万
依托单位：
University of the Ryukyus (1996)Kumamoto University (1995)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

The study was conducted to evaluate the test methods available in Japan to discriminate between the isolates of enterococci resistant to vancomycin (VRE) and those susceptible (VSE). Among the phenotypic test methods evaluated, an agar screening method which utilized 8 mug per ml of vancomycin in Mueller-Hinton agar appeared to have a sufficient accuracy, the results indicating 98.3% of sensitivity and 100% of specificity to detect VRE.Also, all the VRE isolates were interpreted as being resistant or intermediate by the E test when the results were read after 48 hr-incubation. Whereas, two disk diffusion tests, Showa disk and NCCLS-based Sensi-disc, were evaluated, but both failed to discriminate between VRE and VSE,in particular, between the isolates with vanB positive and negative. The automated system, Vitek GPS-TA produced high frequencies of very majior errors ; 8.7% for vanA positives and 58% for vanB positives.A total of 1,214 enterococcal isolates from multisite laboratories in Japan, comprising 7 different species, were first tested onto agar screening test plates, but none of isolates represented phenotypic vancomycin resistance.

本研究旨在评价日本可用的检测方法，以区分对万古霉素耐药（VRE）和敏感（VSE）的肠球菌分离株。在评价的表型试验方法中，在Mueller-Hinton琼脂中使用8 μ g/ml万古霉素的琼脂筛选方法似乎具有足够的准确性，结果表明检测VRE的灵敏度为98.3%，特异性为100%。然而，两种纸片扩散试验，昭和纸片和NCCLS为基础的Sensi-disc，但都不能区分VRE和VSE，特别是与vanB阳性和阴性的分离物。Vitek GPS-TA自动化系统产生了高频率的非常严重的错误; vanA阳性为8.7%，vanB阳性为58%。来自日本多地点实验室的共1，214株肠球菌分离株，包括7个不同的菌种，首先在琼脂筛选试验平板上进行了试验，但没有分离株表现出万古霉素耐药表型。