Quantitative analysis of yeast cell population by using viability-dependent fluorescent dyes and its Clinical application

我们建立了一种用荧光染料直接染色测定尿液标本中酵母细胞活力的方法，荧光染料对酵母细胞的渗透性不同。染色后用流式细胞仪对酵母细胞进行分析。抗感染能力强的患者尿液标本中的酵母细胞大多被归类为损伤细胞，而受损患者尿液标本中的酵母细胞则被归类为活细胞。随着疾病临床表现的改善，损伤细胞比例增加。这一既定的程序为我们提供了一种对各种疾病的预后指标。

We established a technique to determine the viability of yeast cells in urine specimens by directly staining with fluorescent dyes, which have different permeability into yeast cells. After staining, yeast cells were analyzed by flow cytometer. Yeast cells in urine specimens from competent patients against infection were mostly classified as injured cells, whereas those from compromised patients were live cells. Also, the increased ratios of injured cells were observed along the improvement of clinical manifestation of compromised diseases. This established procedure provides us with a perspective prognostic indicator for a variety of diseases following the compromised against infections.