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Effect of UV-B on Rice and Estimation of Its Productivity

UV-B 对水稻的影响及其产量估算

基本信息

批准号：
07680590
负责人：
TAKAYANAGI Shinnosuke
金额：
$ 1.47万
依托单位：
TOHO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680590/
关键词：
Rice Ultra Violet UV-B DNA Damage Photoreactivation Excision Repair Gel Image Analysis UV-Resistance 紫外線感受性 CPD(シクロピリミヂンダイマー)内因性DNA損傷量

项目摘要

DNA damage in two strains of rice by irradiation of UV-B and its ability of repair were searched by alkaline agarose gel electrophoresis and its image analysis system. Compare of CPD (cyclobutyl pyrimidine dimmer) in DNA formed by UV-B irradiation was very similar in the two strains, which were sensitive (Oryza sativa var.Serjumki) and resistant (Oryza sativa var.Marithbach sc-6) against UV.However, their abilities of photoreactivation and excision repair were different. UV-resistant strain showed high abilities of photoreactivation and excision repair, although UV-sensitive one was comparatively weak ability of repair system. While the photoreactivation quickly responded to formation of the CPD,the excision repair system was very slow response. Those results showed that UV-resistance of rice was originated high ability of repair systems.DNA damage, abilities of photoreactivation and excision repair at the 1-2 leaves, 3-4 leaves, and 5-6 leaves stage of rice were searches with measurement of their CPD.Their results showed that stages of 1-2 and 3-4 leaves stage of the rice were very similar ability, although DNA damage and their abilities of repair in rice of 5-6 leaves stage was diminish of about 20%. The reason was thought that UV-B reaching to nucleus in the cell was reduced, as rice at this stage contained many chloroplasts and became to be thick and rigid. It is necessary to search the ability against UV-B in rices cultivated in Japan.

用碱性琼脂糖凝胶电泳法及其图像分析系统研究了UV-B辐射对两个水稻品系的DNA损伤及其修复能力。UV-B辐射产生的DNA中CPD(环丁基嘧啶二聚体)的比较在对紫外线敏感和抗性的两个品系中非常相似，但它们的光复活和切除修复能力不同。耐紫外线菌株表现出较高的光复活和切除修复能力，而对紫外线敏感的菌株修复系统能力相对较弱。虽然光复活对CPD的形成反应迅速，但切除修复系统的反应很慢。对水稻1-2叶期、3-4叶期和5-6叶期的DNA损伤、光复活和切割修复能力进行了测定，结果表明，1-2叶期和3-4叶期的DNA损伤和修复能力非常相似，而5-6叶期的DNA损伤和修复能力要小20%左右。其原因是由于水稻在这个阶段含有大量的叶绿体，变得厚实而僵硬，到达细胞核的UV-B减少了。因此，有必要对日本栽培水稻进行抗UV-B能力的研究。