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Study on signals involved in the cis-trans interactions for S phase-specific transcription in plants

植物S期特异性转录顺反相互作用信号的研究

基本信息

批准号：
07680738
负责人：
IWABUCHI Masaki
金额：
$ 1.47万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680738/
关键词：
S phase transcription Histone H3 promoter Type I element Hex motif Oct motif Transformed cell Transgeneic plant Meristem-specific expression 発生過程転写因子トランスジェニックタバコエフェクター遺伝子 Ti-プラスミド 35Sプロモーター HBP-1a(17)プロトプラスト

项目摘要

The present study aimed to etucidate what is the role of the type I element involved in the S phase-specific transcription regulation of wheat histone genes during the development. The results obtained in the past two years are summarized as follows.1) Structural comparisons of the type I,II and III elements and their functional analysis in stable transformed cells : The cis-elements having the same function as the type I element, consisting of the Hex and Oct motifs, were found. The cis-elements are termed type II and III elements, and both of which contain the Oct motif as well as the type I element. These three elements were shown to have another characteristic motif in addition to the Oct motif, and the cooperative action between two motifs in each element was indispensable for S phase-specific activity of the histone promoter. From the results together with our previous ones, it has been postulated that the Hex motif-specific DNA binding proteins, HBP-1a and-1b, may function to recruit an OBRF on the Oct motif.2) Functional analysis of the type I element in transgenic tobacco plants : To investigate the function of the type I element during the development of plants, we examined whether or not there is any difference in promoter activity between the CaMV35S minimal promoter with the type I element was inactive in the main root although active in the lateral roots and shoot apex. This suggests that the type I element must cooperate with other cis-elements to confer meristem-specific activity to the H3 promoter.

本研究旨在阐明小麦组蛋白基因S期特异性转录调控中I型元件的作用。现将近两年来的研究结果总结如下：1）I、II、III型元件的结构比较及其在稳定转化细胞中的功能分析：发现了与Ⅰ型元件功能相同的由Hex和Oct基序组成的顺式元件，称之为Ⅱ型和Ⅲ型元件，并且两者都含有Oct基序以及I型元件。这三个元件除了Oct基序外还具有另一个特征基序，并且每个元件中两个基序之间的协同作用对于组蛋白启动子的S期特异性活性是必不可少的。根据这些结果以及我们以前的结果，我们推测Hex基序特异性DNA结合蛋白HBP-1a和HBP-1b可能具有在Oct基序上募集OBRF的功能。2）转基因烟草植物中I型元件的功能分析：为了研究I型元素在植物发育过程中的作用，我们检查了CaMV 35 S最小启动子与I型元件之间启动子活性是否有任何差异，I型元件在主根中无活性，但在侧根和茎尖中有活性。这表明I型元件必须与其他顺式元件合作以赋予H3启动子分生组织特异性活性。