Transcription Signals and High-ordered Regulatory Mechanisms for Plant Gene Expression

植物基因表达的转录信号和高阶调控机制

• 批准号: 02304006
• 负责人: IWABUCHI Masaki
• 金额: $ 16万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304006/
• 关键词: Plant genes; Transcription Regulation; Cis-elements; DNA-binding proteins; Cell function; Highly ordered regulation mechanism; Promoter structure; Gene expression specificity; 高次調節機溝; 植物遺伝子転写制御; トランス制御因子; 細胞特異的遺伝子発現; 胚発生; DNA結合タンパク質; 葉緑体遺伝子

The aim of the present cooperation research (A) was to investigate the regulatory mechanisms of plant gene expression though the interaction between cis-acting DNA sequences and trans-control factors. Each partaker used his own experimental system and has identified gene-specific regulatory DNA sequences and the sequence-specific DNA binding proteins.The cis-elements defining the S phase-specific expression of wheat H3 gene were indentified to be the hexameric and octameric motifs in the proximal promoter region and the hexamer-specific DNA-binding protein, HBP-1a(17), was shown to be a bZIP-type protein by sequence analysis of the cDNA clone. The major cis-regions of a concanavalin A gene of Canavalina gladiata was shown to be located downstream of -745 and some DNA-binding proteins that can interact with the regions were also identified. The DNA sequences conferring the induction of high-level of expression of a sweet potato alpha-amylase and sporamin genes was identified. The DNA-binding factor SP8BF was demonstratedto bind to a CGTCA motif in the 5'-upstream region of the alpha-amylase gene. One of the auxin-induced genes, par gene, was isolated and the positive or negative cis-regions were found. DNA-binding proteins that bind to the cis-regions of rolC gene in Ri plasmid were identified.

本合作研究(A)的目的是通过顺式作用的DNA序列和反式调控因子之间的相互作用来研究植物基因表达的调控机制。每个参与者使用自己的实验系统，鉴定了基因特异的调控序列和序列特异的DNA结合蛋白。通过对克隆的序列分析，确定小麦H3基因S阶段特异性表达的顺式元件为近端启动子区域的六聚体和八聚体基序，六聚体特异的DNA结合蛋白HBP-1a(17)为bZIP型蛋白。刀豆蛋白A基因的主要顺式区域位于-745的下游，并鉴定了一些能与这些区域相互作用的DNA结合蛋白。鉴定了诱导甘薯α-淀粉酶和孢子胺基因高水平表达的DNA序列。DNA结合因子SP8BF与α-淀粉酶基因5‘上游区的CGTCA基序结合。分离了生长素诱导基因之一PAR基因，并发现了正负顺式区域。鉴定出了与Ri载体中RolC基因顺式区域结合的DNA结合蛋白。

项目成果

Shimoda,Nobuyoshi: "Control of expression of Agrobacterium tumefaciens vir genes by synergistic actions of phenolic signal and monosaccharides" Proc.Natl.Acad.Sci.USA. 87. 6684-6688 (1990)

Shimoda，Nobuyoshi：“通过酚信号和单糖的协同作用控制根癌农杆菌 vir 基因的表达”Proc.Natl.Acad.Sci.USA。

Sasaki,Yukiko: "Correlation of plastid DNA copy number with plastid gene expression in various organs in mature pea plants(<Pisum>___ー <sativum>___ー L.)" Plant Cell Physiol.31. 925-931 (1990)

Sasaki，Yukiko：“成熟豌豆植物各个器官中质体 DNA 拷贝数与质体基因表达的相关性（<Pisum>____ <sativum>____ L.）” Plant Cell Physiol.31（1990）。 ）

Nagano,Yukio: "An essential gene of <Escherichia>___ー <coli>___ー that has sequence similarity to an unknown chloroplast gene" Mol.Gen.Genet.(1991)

Nagano, Yukio：“<埃希氏菌>___－<大肠杆菌>___－的必需基因，与未知叶绿体基因具有序列相似性”Mol.Gen.Genet.(1991)

町田 泰則(分担執筆): "高等植物の情報発現と制御(岡田・池田編)" 丸善株式会社, 292 (1990)

Yasunori Machida（撰稿人）：“高等植物中的信息表达和控制（冈田和池田编辑）” Maruzen Co., Ltd.，292（1990）

Tabata,T.: "HBPー1a and HBPー1b: Leucineーzipper type transcription factors of wheat" The EMBO J.19. 1459-1467 (1991)

Tabata, T.：“HBP-1a 和 HBP-1b：小麦的亮氨酸拉链型转录因子”EMBO J.19 1459-1467 (1991)。