Gene activation of CREB by light/dark cycle in the rat retina

大鼠视网膜光/暗循环激活 CREB ​​基因

• 批准号: 07680831
• 负责人: IMAKI Junko
• 金额: $ 1.47万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680831/
• 关键词: retina; CREB; in situ hybridization; c-fos mRNA; immunocytochemistry; phosphorylation; rat; Ca^<2+>; カルシウム イオン; 燐酸化CREB; ウエスタンブロット

The signal pathway of the light-induced gene expression in retinal cells was investigated. Flashing light induced c-fos and somatostatin in the cells of the inner border of the inner nuclear layr (INL) and the ganglion nuclear layr (GNL). As both c-fos and somatostatin genes have cAMP responsive element (CRE) in their promoters, CREB phosphorylation in retinal cells was examined with phospho-CREB specific antibody. One min exposure to flashing light induced maximum phosphorylation of CREB showing close correlation with c-fos and somatostatin mRNA expression in the same portion of the retina. Administration of L-type Ca^<2+> channel activator Bay K 8644 also induced CREB phosphorylation and c-fos and somatostatin expression in the rat retina. The distribution of calmodulin kinase II immunoreactive retina cells was in good accordance with the phospho-CREB immunoreactivity. Thus we propose that transcriptional response to light stimulation is regulated via CREB phosphorylation catalyzed by Ca^<2+>/calmodulin-dependent protein kinase in neural retina.

研究了视网膜细胞中光诱导基因表达的信号通路。闪光诱导内核层（INL）和神经节核层（GNL）内缘细胞中的c-fos和生长抑素。由于c-fos和生长抑素基因的启动子中都有cAMP反应元件(CRE)，因此用磷酸CREB特异性抗体检查视网膜细胞中的CREB磷酸化。暴露于闪光一分钟诱导 CREB ​​的最大磷酸化，显示与视网膜同一部分中的 c-fos 和生长抑素 mRNA 表达密切相关。 L-型Ca 2+ 通道激活剂Bay K 8644的施用还诱导大鼠视网膜中的CREB磷酸化以及c-fos和生长抑素表达。钙调蛋白激酶 II 免疫反应性视网膜细胞的分布与磷酸化 CREB ​​免疫反应性良好一致。因此，我们提出对光刺激的转录反应是通过神经视网膜中Ca 2+ /钙调蛋白依赖性蛋白激酶催化的CREB磷酸化来调节的。

项目成果

Harada,T: "Cone-associated c-fos gene expression in the light-damaged rat retina" Invest.Ophtalmol Visual Sci. 37. 1250-1255 (1996)

Harada,T：“光损伤大鼠视网膜中视锥相关的 c-fos 基因表达”Invest.Ophtalmol Visual Sci。

Yoshida, K., Imaki, J., Fujisawa, H., Harada, T., Ohki, K., Matsuda, H.and Hagiwara, M.: "Defferential distribution of CaM kinases and induction of c-fos expression by flashing and sustained light in rat retinal cells" Investigative Ophthalmology & Visual

Yoshida, K.、Imaki, J.、Fujisawa, H.、Harada, T.、Ohki, K.、Matsuda, H. 和 Hagiwara, M.：“CaM 激酶的差异分布和通过闪烁诱导 c-fos 表达

Harada,T: "Cone-associated c-fos gene expression in the light-damaged rat retinal" Invest.Ophtalmol Visual Sci. 37. 1250-1255 (1996)

Harada,T：“光损伤大鼠视网膜中视锥相关的 c-fos 基因表达”Invest.Ophtalmol Visual Sci。

Hagiwara,M.: "Intracellular Signal Transduction" Academic Press Inc., 310 (1996)

Hagiwara,M.：“细胞内信号转导”Academic Press Inc.，310（1996）

Imaki,J.: "Expression of jun family genes in rat retinal cells regulation by light/dark cycle" Molecular Brain Research. 30. 48-52 (1995)

Imaki,J.：“jun 家族基因在大鼠视网膜细胞中通过光/暗循环调节的表达”分子脑研究。