Circadian ocillation and mechanisms of c-fos mRNA in rat cortex

大鼠皮层c-fos mRNA的昼夜节律振荡及机制

• 批准号: 10680712
• 负责人: IMAKI Junko
• 金额: $ 1.86万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680712/
• 关键词: c-fos; mRNA; neocortex; propofol; circadian rhythm; rat; in situ hybridization; midline thalamic nuclei; 日内変動; プロポフォール; C-fos; 大脳新皮質

c-fos gene is one of the oncogene which is responsible by many stimulus and is inducible. c-fos was reported inducing in neurons by depolarized with Ca influx or increasing of cAMP. We reported that it's expression showed circadian rhythm in the rat cortex. And the expression showed 2 different patterns. Then We investigated intravenous anesthetic Propofol (2, 6-di-isopropylpenol) suppressed the induction of c-fos mRNA in the rat neocortex which expression began to increase 2 hours before the dark period and remained elevated following the dark period in a normal rat. Simultaneously, administration of Propofol induce the expression of c-fos mRNA in the midline thalamic nuclei which is also activated by some benzodiazepine narcotic drugs. These indicate that narcotic action of Propofol will exhibit via activation of midline thalamic nuclei. We also investigate that c-fos mRNA expression in the olivary nucleus and cereberal cortex in administration of Propofol at the rate of 20 mg/kg or 30 mg/kg. Rate of 10 mg/kg do not induce the c-fos mRNA in these nucleus and cortex, suggest that comparative high dose administration of propofol have some affects for these nucleus.

C-fos基因是受多种刺激作用的可诱导致癌基因之一。c-fos是通过Ca内流去极化或cAMP升高诱导的。我们报道了它在大鼠皮层的表达具有昼夜节律。表达式呈现出两种不同的模式。然后我们研究了静脉麻醉丙酚（2,6 -二异丙醇）对大鼠新皮层c-fos mRNA表达的抑制作用，在正常大鼠黑暗期前2小时c-fos mRNA表达开始增加，在黑暗期后继续升高。同时，异丙酚可诱导丘脑中线核c-fos mRNA的表达，该mRNA也可被苯二氮类药物激活。这表明异丙酚的麻醉作用是通过激活丘脑中线核来表现的。我们还研究了丙泊酚在20 mg/kg或30 mg/kg剂量下对橄榄核和大脑皮层c-fos mRNA表达的影响。10 mg/kg剂量的异丙酚对大鼠核和皮层的c-fos mRNA表达无诱导作用，提示相对高剂量异丙酚对大鼠核和皮层的c-fos mRNA表达有一定影响。

项目成果

Yoshida K, Nakayama K, Nagahama H, Harada T, Harada C, Imaki J, Matsuda A, Yamamoto K, Ito M, Ohno S, Nakayama K.: "Involvement of p27(KIP1) degradation by Skp2 in the regulation of proliferation in response to wounding of corneal epithelium"Invest Ophtha

Yoshida K、Nakayama K、Nagahama H、Harada T、Harada C、Imaki J、Matsuda A、Yamamoto K、Ito M、Ohno S、Nakayama K.：“Skp2 对 p27(KIP1) 降解参与增殖调节

Sakai M.: "Regulation of c-maf gene expression by Pax 6 in culture cells"Nucleic. Acid Res. 13. 328-338 (2001)

Sakai M.：“培养细胞中 Pax 6 对 c-maf 基因表达的调节”Nucleic。

Yamada K, Wada E, Imaki J. Ohki-Hamazaki H, Wada K.: "Hyperresponsiveness to palatable and aversive taste stimuli in genetically obese (bombesin receptor subtype-3-deficient) mice"Physiol. Behav.. 66. 863-867 (1999)

Yamada K、Wada E、Imaki J. Ohki-Hamazaki H、Wada K.：“遗传性肥胖（铃蟾肽受体亚型 3 缺陷）小鼠对可口和厌恶味觉刺激的过度反应”Physiol。

Imaki, J.: "Developmental expression of maf-1 messenger ribonucleic acids in rat kidney by in situ hybridization histochemistry"BBRC. 272. 777-782 (2000)

Imaki, J.：“通过原位杂交组织化学研究大鼠肾脏中 maf-1 信使核糖核酸的发育表达”BBRC。

Kawahara H.: "A Prostaglandin E2 receptor subtype EP1 receptor antagonist(ONO-8711) reduces hyperalgesia, allodynia and c-fos gene"Anesth Analg. 93. 1012-1017 (2001)

Kawahara H.：“前列腺素 E2 受体亚型 EP1 受体拮抗剂 (ONO-8711) 可减少痛觉过敏、异常性疼痛和 c-fos 基因”Anesth Analg。