Preparation and evaluation of calcium phophate porous particles for drug carrier

药物载体磷酸钙多孔颗粒的制备及评价

• 批准号: 07680935
• 负责人: OHGAKI Masataka
• 金额: $ 1.41万
• 依托单位: Tokyo medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680935/
• 关键词: drug carrier; calcium phosphate; porous particle; apatite; TCP; 多孔粉体; 微粒子; 水酸アパタイト

Hydroxyapatite (HAp), cation substituted HAp particles, and alpha-tricalcium phosphate (alpha-TCP) particles were synthesized. Characterization of these particles were examined by X-ray diffraction, IR and SEM techniques, and by measurements of particle size distributions and Zeta potential. Cytotoxicities of particles were evaluated by cell culture techniques for HAp and alpha-TCP coating layrs on Ti plate.The particles prepared with properly sizes and heated with properly temperatures were requisite to optimize porous HAp and alpha-TCP powders used for drug carrier. Effects of Zeta potential behavior indicated that the isostatic points of HAp particles were shifted toward negatively charged in proportion to evaluating at heating temperature. The alpha-TCP powders prepared by wet method contained carbonate ions, and that prepared by plasma techniques had broad X-ray diffraction peaks of less crystallinity. Prolification curves and growth rates of cells cultured with HAp and alpha-TCP indicated that the HAp and alpha-TCP had good biocompatibility.Newly synthesized porous alpha-TCP of 100mum spherical particles had a large number of open-pores of 2-10mum in diameter. These particles are useful for drug carriers as various drug derivery system.

合成了羟基磷灰石（HAp）、阳离子取代的HAp颗粒和α-磷酸三钙（α-TCP）颗粒。通过X射线衍射、红外光谱、扫描电镜、粒度分布和Zeta电位的测定，研究了这些粒子的表征。通过细胞培养技术对HA和α-TCP涂层颗粒的细胞毒性进行了评价，结果表明，制备出的HA和α-TCP涂层颗粒具有良好的尺寸和加热温度，是优化HA和α-TCP多孔粉末作为药物载体的必要条件。Zeta电位行为的影响表明，HAp颗粒的平衡点移向负电荷的比例在加热温度下进行评估。湿法制备的α-TCP粉末含有碳酸根离子，而等离子体技术制备的α-TCP粉末具有较宽的X射线衍射峰，结晶度较低。用HAp和α-TCP培养的细胞增殖曲线和生长速率表明HAp和α-TCP具有良好的生物相容性，新合成的多孔α-TCP为100 μ m球形颗粒，具有大量直径为2- 10 μ m的开孔。这些颗粒可用作各种药物衍生系统的药物载体。