浮遊性かいあし類における化学物質による休眠卵産生の誘因

浮游桡足类滞育产卵的化学触发因素

• 批准号: 07760175
• 负责人: 伴 修平
• 金额: $ 0.51万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07760175/
• 关键词: かいあし類; Eurytemora affinis; 休眠卵; 休眠誘発; 代謝物質; 込み合い効果

休眠卵から飼育した浮遊性かいあし類Eurytemora affinisの後期ノ-プリウス幼生3,000個体を10mlの蒸留水中(1)で、また成体400-800個体を20mlの蒸留水中(2)でそれぞれすり潰し、4,500rpmで10分間遠心分離し、その上澄みをかいあし類抽出液とした。実験には、400mlのガラス瓶を用い、濾過湖水に餌としてCryptomonas tetrapyrenoidosaを充分量加えたものを飼育水とした。これに、孵化直後のノ-プリウス幼生50個体を入れ、先のかいあし類抽出液を適宜加え、3-4日間隔で飼育水を交換した。なお、新しい飼育水に交換した際には、再びかいあし類抽出液を加えた。対照区としては、抽出液と同量の濾過湖水を加えた。対照区と、ノ-プリウス幼生からの抽出液(1)ではどちらも全く休眠卵を作らなかったのに対して、成体からの抽出液(2)では90%以上の雌が休眠卵を作った。これらの結果は、コペポ-ダの体成分のうち水溶性の物質が明らかに休眠卵産生を誘引することを証明した。また、一度に加えた抽出液は、乾重量に換算すると、ノ-プリウス幼生からのものが0.12mgであるのに対して、成体からのものが0.32mgであり、(1)と(2)の結果の違いは、おそらく抽出液中の誘引物質の濃度に依存したためと考えられる。

In the later stage of Eurytemora affinis of resting eggs and resting eggs, 3000 larvae of resting eggs and 3000 adults of 10ml remained in the water (1), and in the water of adults (4-800). The samples were extracted from the water after 10 minutes of incubation, and the samples were separated at 10 minutes of 4500rpm, and then extracted from the water. Make sure that the water bottle is used, and that the Cryptomonas tetrapyrenoidosa is used in the water of the lake, and that the water is nurtured in a full amount. After hatching and hatching, 50 young babies were admitted, the extraction fluid of the first batch should be added, and the water samples should be reared at intervals of 3-4 days. The new water supply system is used to increase the volume of the liquid extracted from the water supply system, such as the international water supply system and the water supply system. In the illuminated area, the amount of water extracted is the same as that of the water in the lake. The young were exposed to the extract (1), and the whole resting egg was used as the egg extract, and the adult extract (2) was used as the female resting egg extract. The results showed that the water-soluble substances were not soluble in water-soluble substances, and the dormant eggs were born in the first place. The results showed that the concentration of primers in the extract was significantly higher than that in the extract, the concentration of primers in the extract was significantly higher than that in the extract, the concentration of the primers in the extract was much higher than that in the extract, the concentration of the primers in the extract was much higher than that in the extract, the concentration of the primers in the extract, the concentration of primers in the extract, the concentration of the primers in the extract, the concentration of primers in the extract, the