血小板がmechanical lung Injuryに及ぼす影響

血小板对机械性肺损伤的影响

• 批准号: 07770414
• 负责人: 中野 剛
• 金额: $ 0.64万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07770414/
• 关键词: 摘出潅流肺 pertused lung madel; 血小板 platelet; mechanical lung injury

【目的】ラット摘出潅流肺モデルにおいて1)潅流域に血小板が存在しない場合,静水圧上昇による肺障害(Mechanical lung injury)が惹起されるか.2)もし惹起されるとすれば,潅流液に血小板を入れるとmechanical lung injuryは抑制されるか.【今年度の実施結果】ラット摘出潅流肺モデルを以下のように作成した.ラット心肺をen blocでとりだし,肺動脈・(左室を通して)左房にカニュレーションを行い,4%bovine serum albumin加EBSS液をもちいZone3の状態で潅流を行った.換気は5%CO2,35%0.2,60%N2の混合ガスを用いて換気回数60回/分,1回換気量3ml,PEEP2cmH20にて行った.潅流液のリザーバーは37℃になるように保温し,潅流肺重量を張力トランスデユーサーを用いて測定した.潅流圧を上昇させるため,肺動脈側,左房側のリザーバーを5cm上昇させ,肺重量の変化を記録した.横軸に時間,縦軸に単位時間あたりの重量変化を常用対数に変換してとると,2相に分別できる.後半を直線回帰し,時間0に外挿し初期濾過率を求め,これより濾過係数を計算した.一つの肺(n=1)について3回繰り返しその平均を求めた.血小板を含まない4%bovine serum albumin加EBSS液を用いた場合,濾過係数は53.2±(SD)5.2mg min^<-1> cmH2O^<-1> g^<-1>(n=5)であった.いずれの場合も,リザーバーの高さをもとに戻すと肺重量はベースラインに戻り測定可能であった.リザーバーの上昇を10cmにしても高さをもとに戻すと肺重量はベースラインに戻った.ところが,リザーバーを20cmに上昇させたのちリザーバーの高さをもとに戻しても肺重量はベースラインに戻らず,肺水腫が惹起された.血小板がない状態では20cmH20の静水圧でMechanical lung injuryが惹起されたと考えられた.今後,潅流液に血小板をいれてMechanical lung injuryは抑制されるか検討予定である.

[objective] to extract the platelets from the watershed. [objective] there is a clot in the platelets of the river basins. 2) the platelets in the still water cause lung damage (Mechanical lung injury). The flow of the platelets into the platelets inhibits the growth of the platelets. [the results of this year's experiment] the results of this year's experiment show that the platelets are extracted from the outflow lungs and the following samples are made. The heart and lungs, the en bloc, the lungs, the left atrium, the left atrium, the lungs, the lungs, the 5% CO2Magi 35% 0.2 Jing 60% N2 mixtures. Please count back 60 times per minute with a mix of peeps 2cmH20. The fluid is heated at 37 ℃ to keep warm, and the weight of the lungs is used to measure the temperature. The flow rate was high, the lung movement was abnormal, the left atrium was damaged, the 5cm was elevated, and the lung weight was recorded. In the middle of the day, there is a difference in the amount of time, the amount of time. In the second half of the year, the last half of the year will be returned straight-line, and the initial overrun rate will be calculated in the second half of the year. One lung (n = 1) has three times to return to the average. The platelet count was 53.2 ±(SD) 5.2mg 4%bovine serum albumin & lt;-1> cmH2O^ & lt;-1> g ^ & lt;-1> (n = 5). The weight of the lungs, the lung weight, lung weight The weight of the lungs, the weight of the lungs, the 10cm, the lungs, the lungs. The weight of the lungs, the weight of the lungs, the lungs. Platelet 20cmH20, still water, Mechanical lung injury, provoke a lot of trouble. From now on, the flow of blood platelets, platelets, Mechanical lung injury suppresses the flow of blood, and predetermines the concentration of blood.