乳癌患者における骨髄または末梢血中癌細胞に対する高感度同定法の有用性

高灵敏鉴定乳腺癌患者骨髓或外周血癌细胞的方法的应用

• 批准号: 07770948
• 负责人: 竹島 薫
• 金额: $ 0.7万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07770948/
• 关键词: Cytokeratin19mRNA; RT-nested PCR; 乳癌細胞高感度検出法; 腫瘍マーカー

Cytokeratin19mRNAに対するRT-nested PCR法を用いた乳癌細胞高感度検出法を開発した.本法の検出感度は血中有核細胞100万個当たり乳癌癌細胞25個であった.正常骨髄を計14例検討したところ7例にCytokeratin19mRNAの発現を認めたが,正常末梢血9例中にはその発現は認めなかったので乳癌患者末梢血検体に対して本法による検討を行いその臨床腫瘍学的意義を検討した.初発乳癌患者58例および再発乳癌患者8例に対し,それぞれ手術前と治療前に末梢血を採血し本法によりCytokeratin19mRNAの発現を検討した.それぞれ14例,6例で発現を認め,正常末梢血を含めた3群間でその発現率に有意差を認めた.また病理組織学所見で血管侵襲群と非侵襲群でCytokeratin19mRNAの発現を比較したところ血管侵襲群で有意に発現率が高かった.以上の結果よりCytokeratin19mRNAに対するRT-PCRを用いて乳癌細胞が鋭敏に検出されることが明かとなり,本法を利用することによって血中乳癌細胞を検出しうること,また血中乳癌細胞が腫瘍マーカーとして有用であることが示唆された.

Cytokeratin19mRNAに対するRT-nested The PCR method is used to extract breast cancer cells with high sensitivity. The sensitivity of this method is to detect 1 million nucleated cells in the blood when 25 breast cancer cells are detected. .Normal bone marrow was found in 14 cases, 7 cases were found to have Cytokeratin19mRNA, and 9 cases were found to have normal peripheral blood. The clinical oncology significance of this method and its significance in clinical oncology are as follows: 58 cases of early-stage breast cancer patients. 8 cases of recurrent breast cancer patients. それぞれBefore surgery and treatment. Collect peripheral blood and collect blood. This method is によりCytokeratin19mRNA and it is now. There were 14 cases in which the disease was detected, and 6 cases had symptoms that were not recognized, and normal peripheral blood was found. There was an intentional difference in the occurrence rate of disease among 3 groups, and the results were recognized by pathological histological findings. Comparison of the appearance of Cytokeratin19mRNA in the vascular invasive group and the non-invasive group. The vascular invasive group has an intentional appearance rate and is high. More thanのRESULTS Cytokeratin19mRNA に対するRT-PCRを Used いて Breast cancer cells が鋭sensitに検出 されることが明かとなり, this How to use the breast cancer cells in the blood and how to use them. Breast cancer cells in the blood are useful in treating breast cancer.