权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study of hematogenous micrometastases by RT-nested PCR of CEA mRNA in patients with esophageal cancer receiving chemoradiotherapy

RT-巢式PCR检测食管癌放化疗患者血行微转移CEA mRNA的研究

基本信息

批准号：
14570886
负责人：
YAMAMICHI Keigo
金额：
$ 2.18万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570886/
关键词：
Esophageal cancer Chemoradiotherapy micrometastasis CEA mRNA RT-PCR

项目摘要

Introduction : Esophageal cancer is a highly virulent disease. Recent reported results of chemoradiotherapy (CRT) have indicated various advantages of this treatment against advanced esophageal cancer. In early stages, definitive CRT as well as surgical resection provide good results. In advanced stages of the disease, neo-adjuvant CRT followed by surgery improve primary tumor resectability and overall survival. However, some patients die due to distant spread shortly after surgery. Those patients may already have had micrometastases before surgical resection. Thus, early detection of micrometastasis is an issue of utmost importance. In this study, we attempted to detect hematogenous micrometastasis by RT-PCR of CEA mRNA in patients with esophageal cancer receiving CRT and evaluated the clinical significance.Methods : A total of 55 esophageal cancer patients who underwent CRT in our university hospital between 2001 and 2004 were studied. Thirty-three of 55 patients received surgery aft … More er CRT. Blood samples were aspirated from peripheral vein at pre- and post-CRT, and post-surgery. After total RNA was extracted from each blood sample, CEA-specific RT-nested PCR was performed. All patients were followed-up for one year.Results : The positive expression of CEA mRNA before CRT was 52.7% (29/55). CEA mRNA before CRT correlated with lymph node metastasis and clinical stage. The number of cases with positive expression of CEA mRNA decreased after CRT (41.8%, 23/55). The expression of CEA mRNA also changed after surgery in 6 patients ; 5 patients became negative and one patient became positive. Expression of CEA mRNA after final treatments correlated recurrence and a poor prognosis.Conclusion : CEA mRNA is a sensitive and specific molecular marker for detection of micrometastasis in peripheral blood of patient with esophageal cancer. It can help to predict the prognosis of esophageal cancer after final treatment, and to determine which patient will benefit from aggressive adjuvant therapies. Less

前言：食管癌是一种高度恶性的疾病。近期报道的放化疗（CRT）的结果表明，这种治疗对晚期食管癌的各种优势。在早期阶段，明确的CRT以及手术切除提供了良好的结果。在疾病的晚期，新辅助CRT加手术可提高原发性肿瘤的可切除性和总生存率。然而，一些患者在手术后不久因远处扩散而死亡。这些患者在手术切除前可能已经有微转移。因此，早期检测微转移是一个至关重要的问题。本研究应用RT-PCR技术检测食管癌放疗患者血行微转移癌胚抗原（CEA）mRNA的表达，探讨其临床意义。55例患者中有33例在术后接受了手术， ...更多信息呃CRT。在CRT前后和手术后从外周静脉抽取血液样品。从每个血液样品中提取总RNA后，进行CEA特异性RT-nested PCR。结果：放疗前癌胚抗原mRNA阳性表达率为52.7%（29/55）。CRT前CEA mRNA表达与淋巴结转移和临床分期有关。CRT后CEA mRNA阳性表达率下降（41.8%，23/55）。6例患者术后CEA mRNA表达也发生变化，5例患者术后CEA mRNA表达阴性，1例患者术后CEA mRNA表达阳性。结论：CEA mRNA是检测食管癌患者外周血微转移的敏感、特异的分子标志物。它可以帮助预测最终治疗后食管癌的预后，并确定哪些患者将受益于积极的辅助治疗。少