Development of multi-wavelength absorption spectrometer for plant by using modulation method

调制法植物多波长吸收光谱仪的研制

• 批准号: 07554069
• 负责人: TAKABE Teruhiro
• 金额: $ 0.32万
• 依托单位: Meijo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07554069/
• 关键词: photosynthesis; environmental responses; nondestructive measurement; transgenic plant; food crisis; fluorescennce; 変調分光; 電子伝達

Recently, molecular biological techniques are well developed and used to make transgenic plants. However, physiological analyzes of transgenic plants are suffered due to the lack of in vivo measurement system. Therefore, we have developed the multi-wavelength absorption spectrometer for plant by using modulation methods. These techniques were used to uncover the adaptation mechanisms of photosystems for halotolerance. Photoacoustic measurements indicated that appreciable energy storage by photosystem I mediated cyclic electron flow at high salinities. Significant electron donation to photosystem I reaction centers through NAD (P) H-dehydrogenase complexes was observed in high salt media. The contents of Cyt.b6/f and PS II were almost constant under various salinity conditions, whereas the levels of Chl a, PS I,soluble Cyt.c-553, and NAD (P) H-dehydrogenase increased in the cells grown with high salinitie, indicating the improtance of cyclic electron flow around PS I for the adaptation to salt stress. The modulated multiwavelength florometer was als used for the analyzes of photosynthesis in transgenic plants.

近年来，分子生物学技术得到了很好的发展，并用于转基因植物的制备。然而，由于缺乏活体检测系统，转基因植物的生理分析受到了限制。为此，我们研制了一种采用调制方法的多波长植物吸收光谱仪。这些技术被用来揭示光系统对盐胁迫的适应机制。光声测量表明，可观的能量存储由光系统I介导的循环电子流在高盐度。在高盐介质中，通过NAD（P）H-脱氢酶复合物观察到了对光系统I反应中心的显着电子捐赠。在不同盐度条件下，Cyt.b6/f和PS Ⅱ的含量基本保持不变，而在高盐度条件下，叶绿素a、PS Ⅰ、可溶性Cyt.c-553和NAD（P）H-脱氢酶的含量增加，表明PS Ⅰ周围的循环电子流对盐胁迫的适应起重要作用。将调制多波长荧光计用于转基因植物的光合作用分析。

项目成果

T.Hibino,B.H.Lee,A.K.Rai,H.Ishikawa,H.Kojima,M.Tawada,H.Shimoyama,T.Takabe: "Salt Enhances Photosystem I Content and Cyclic Electron Flow via NAD (P) H Dehydrogenase in the Halotolerant Cyanobacterium Aphanothece halophytica" Aust J. Plant Physiol.23. 321

T.Hibino,B.H.Lee,A.K.Rai,H.Ishikawa,H.Kojima,M.Tawada,H.Shimoyama,T.Takabe：“盐通过 NAD (P) H 脱氢酶增强耐盐环境中的光系统 I 含量和循环电子流

B.H., Lee, T.Hibino, T.Takabe, P.J.Weisbeek, & T.Takabe: "Site-directed Mutagenetic Study on the Role of Negative Patches on Silene Plastocysanin in the Interactions with Cytochrome f and Photosystem I." J.Biochem.117. 1209-1217 (1995)

B.H.、李、T.Hibino、T.Takabe、P.J.Weisbeek、

M.Nomura, M.Ishitani, T.Takabe, A.K.Rai, and T.Takabe: "Synechococcus sp.PCC7942 Transformed with Escherichia coli bet Genes Produces Betaine and Aquires Resistance to Salt stress." Plant Physiol.104. 703-708 (1995)

M.Nomura、M.Ishitani、T.Takabe、A.K.Rai 和 T.Takabe：“用大肠杆菌 bet 基因转化的聚球藻 sp.PCC7942 产生甜菜碱并获得对盐胁迫的抵抗力。”

T.Nakamura, M.Ishitani, P.Harinasut, M.Nomura, T.Takabe and T.Takabe: "Distribution of Glycinebetaine in Old and Young Leaf Blades of Salt Stressed Barley Plants(1996)" Plant Cell Physiol.37. 873-877 (1996)

T.Nakamura、M.Ishitani、P.Harinasut、M.Nomura、T.Takabe 和 T.Takabe：“盐胁迫大麦植物老叶和幼叶中甘氨酸甜菜碱的分布（1996）”植物细胞生理学.37。

T. Hibino, B. H., Lee, T. Takabe, & T. Takabe: "Expression and Characterization of Met92Gln Mutant Plastocyanin from Silene pratensis." J. Biochem.117. 101-106 (1995)

T. Hibino, B. H., Lee, T. Takabe,