Development of multi-wavelength absorption spectrometer for plant by using modulation method

近年来，分子生物学技术得到了很好的发展，并被用于制造转基因植物。然而，由于缺乏体内测量系统，转基因植物的生理分析受到了影响。为此，我们采用调制方法研制了植物多波长吸收光谱仪。这些技术被用来揭示光系统对耐盐性的适应机制。光声测量表明，在高盐度条件下，光系统I介导的循环电子流具有可观的能量储存。在高盐介质中，通过NAD (P) h -脱氢酶配合物向光系统I反应中心提供了大量电子。Cyt的含量。b6/f和PS II在不同盐度条件下基本保持不变，而Chl a、PS I、可溶性Cyt.c-553和NAD (P) h -脱氢酶的水平在高盐度条件下增加，说明PS I周围的循环电子流对适应盐胁迫的重要性。调制多波长测花仪也被用于转基因植物光合作用的分析。

Recently, molecular biological techniques are well developed and used to make transgenic plants. However, physiological analyzes of transgenic plants are suffered due to the lack of in vivo measurement system. Therefore, we have developed the multi-wavelength absorption spectrometer for plant by using modulation methods. These techniques were used to uncover the adaptation mechanisms of photosystems for halotolerance. Photoacoustic measurements indicated that appreciable energy storage by photosystem I mediated cyclic electron flow at high salinities. Significant electron donation to photosystem I reaction centers through NAD (P) H-dehydrogenase complexes was observed in high salt media. The contents of Cyt.b6/f and PS II were almost constant under various salinity conditions, whereas the levels of Chl a, PS I,soluble Cyt.c-553, and NAD (P) H-dehydrogenase increased in the cells grown with high salinitie, indicating the improtance of cyclic electron flow around PS I for the adaptation to salt stress. The modulated multiwavelength florometer was als used for the analyzes of photosynthesis in transgenic plants.