Development of multi-wavelength absorption spectrometer for plant by using modulation method

近年来，分子生物学技术得到了很好的发展，并用于转基因植物的制备。然而，由于缺乏活体检测系统，转基因植物的生理分析受到了限制。为此，我们研制了一种采用调制方法的多波长植物吸收光谱仪。这些技术被用来揭示光系统对盐胁迫的适应机制。光声测量表明，可观的能量存储由光系统I介导的循环电子流在高盐度。在高盐介质中，通过NAD（P）H-脱氢酶复合物观察到了对光系统I反应中心的显着电子捐赠。在不同盐度条件下，Cyt.b6/f和PS Ⅱ的含量基本保持不变，而在高盐度条件下，叶绿素a、PS Ⅰ、可溶性Cyt.c-553和NAD（P）H-脱氢酶的含量增加，表明PS Ⅰ周围的循环电子流对盐胁迫的适应起重要作用。调制多波长荧光计还用于转基因植物光合作用的分析。

Recently, molecular biological techniques are well developed and used to make transgenic plants. However, physiological analyzes of transgenic plants are suffered due to the lack of in vivo measurement system. Therefore, we have developed the multi-wavelength absorption spectrometer for plant by using modulation methods. These techniques were used to uncover the adaptation mechanisms of photosystems for halotolerance. Photoacoustic measurements indicated that appreciable energy storage by photosystem I mediated cyclic electron flow at high salinities. Significant electron donation to photosystem I reaction centers through NAD (P) H-dehydrogenase complexes was observed in high salt media. The contents of Cyt.b6/f and PS II were almost constant under various salinity conditions, whereas the levels of Chl a, PS I,soluble Cyt.c-553, and NAD (P) H-dehydrogenase increased in the cells grown with high salinitie, indicating the improtance of cyclic electron flow around PS I for the adaptation to salt stress. The modulated multiwavelength florometer was als used for the analyzes of photosynthesis in transgenic plants.