Assembly of Photosynthetic Electron Transport Systems

光合电子传输系统的组装

• 批准号: 01540569
• 负责人: TAKABE Teruhiro
• 金额: $ 1.28万
• 依托单位: Meijo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540569/
• 关键词: Light-Energy; Gene Expression; Photosynthesis; Electron Transfer; Crop

1. Structure and expression of genes the which encode P700-chlorophyll a protein complex---(1) A cDNA clone encoding the polypeptide from cucumber PSI (P700-chlorophyll a protein) complex that migrates with apparent molecular weight of 20 kDa on SDS-polyacrylamide gels has been isolated. The 907-bp sequence of this clone has been determined and contains one open reading frame that encodes a 22, 720-Da precursor polypeptide. The molecular weight of the mature polypeptide was predicted to be 17, 037-Da. The polypeptide was identified as the PsaD gene product. The biogenesis of this polypeptide was also studied. These results were published as a paper : Plant Cell Physiol. 31, 871-879, 1990.(2) Structural and functional aspects of cucumber PSI complexes have been studied. The PSI complex which contains eight polypeptides with apparent molecular masses of 65/63, 20, 19.5, 18.5, 17.5, 7.6, and 5.8 kDa, has been shown to catalyze the light-dependent transfer of electrons from plastocyanin to … More ferredoxin. It was found that the amount of 18.5 kDa-polypeptide is sensitive for the activity. The 18.5 kDa-polypeptide was identified as PsaF gene product and plastocyanin docking protein. A paper describing these results was submitted.(3) The N-terminal amino acid sequencing was carried out to characterize the genes of cucumber PSI complex that catalyzes the light-dependent transfer of electrons from plastocyanin to ferredoxin. A paper describing these results is in the press.2. Assembly of pigments-protein complexes during greening---The relationship between the accumulation of chlorophyll, heme and polypeptides during greening of cucumber cotyledons was studied. It was found that distribution of newly synthesized chlorophyll among apo-proteins is regulated by the relative rates of synthesis of chlorophyll a and apo-proteins as well as the differential affinities of the apo-proteins for chlorophyll a but not chlorophyll b. These results were published as a paper : Plant Cell Physiol. 31, 639-647, 1990.3. Expression of precursor plastocyanin in E. coli and reconstitution of mature plastocyanin---The precursor plastocyanin was expressed in E. coli and partially purified. The precursor plastocyanin was processed in vitro into the mature apo-plastocyanin. Copper atom was incorporated into the apo-protein the and resulting holo-protein exhibited the same activity for the electron transfer with the native purified plastocyanin. A paper describing these results is in the press. Less

1. P700-叶绿素a蛋白复合物基因的结构与表达（1）从黄瓜PSI（P700-chlorophylla protein）复合物中分离到一个编码多肽的cDNA克隆，该多肽在SDS-聚丙烯酰胺凝胶上的表观分子量为20 kDa。该克隆的907-bp序列已被确定，并含有一个编码22，720-Da前体多肽的开放阅读框。预测成熟多肽的分子量为17，037-Da，鉴定为PsaD基因产物。还研究了这种多肽的生物发生。这些结果作为论文发表：Plant Cell Physiol.31，871-879，1990。(2)黄瓜PSI复合物的结构和功能方面进行了研究。PSI复合物含有表观分子量为65/63、20、19.5、18.5、17.5、7.6和5.8 kDa的8种多肽，已显示其催化电子从质体蓝蛋白到质体蓝蛋白的光依赖性转移。 ...更多信息 铁氧还蛋白。发现18.5kDa多肽的量对活性敏感。经鉴定，该多肽分子量为18.5kDa，为PsaF基因产物和质体蓝素对接蛋白。提交了一份描述这些结果的文件。(3)对黄瓜PSI复合物的N-末端氨基酸序列进行了分析，并对PSI复合物中光依赖性电子从质体蓝蛋白到铁氧还蛋白的传递进行了研究。描述这些结果的论文已在印刷中。绿色化过程中色素-蛋白质复合物的组装--研究了黄瓜子叶绿色化过程中叶绿素、血红素和多肽积累的关系。结果表明，新合成的叶绿素在脱辅基蛋白中的分布受叶绿素a和脱辅基蛋白合成的相对速率以及脱辅基蛋白对叶绿素a的不同亲和力的调节，而对叶绿素B则无影响。这些结果作为论文发表：Plant Cell Physiol.31，639-647，1990.3。质体蓝素前体在大肠杆菌中的表达。大肠杆菌中表达了成熟的质体蓝素前体蛋白--质体蓝素前体蛋白。大肠杆菌并部分纯化。在体外将前体质体蓝素加工成成熟的脱辅基质体蓝素。将铜原子掺入脱辅基蛋白中，得到的全蛋白具有与天然纯化质体蓝素相同的电子传递活性。一份描述这些结果的论文已经出版。少

项目成果

T.Takabe,T.Hibino,H.Ishikawa,H.Inoue and Y.Iwasaki: "Biosynthesis of Photosystem I and Cytochrome b6/f Complexes" Physiologia Plantarum. 76. 131 (1989)

T.Takabe、T.Hibino、H.Ishikawa、H.Inoue 和 Y.Iwasaki：“光系统 I 和细胞色素 b6/f 复合物的生物合成”Physiologia Plantarum。

Y. Iwasaki, H. Ishikawa, T. Hibino, and T. Takabe: "Characterization of Genes that Encode Subunits of Cucumber PSI Complex by N-Terminal Sequencing" Biochim. Biophys. Acta. (1991)

Y. Iwasaki、H. Ishikawa、T. Hibino 和 T. Takabe：“通过 N 端测序对编码黄瓜 PSI 复合体亚基的基因进行表征”Biochim。

T.Takabe,Y.Iwasaki,T.Hibino,and T.Ando: "Subunit Composition of Photosystem I Complex that Catalyzes LightーDependent Transfer of Electrons from Plastocyanin to Ferredoxin" Biochim.Biophys.Acta. (1991)

T.Takabe、Y.Iwasaki、T.Hibino 和 T.Ando：“催化光依赖性电子从质体蓝素转移到铁氧还蛋白的光系统 I 复合体的亚基组成”Biochim.Biophys.Acta。

Y.Iwasaki,T.Sasaki and T.Takabe: "Sequencing and Expression of the Gene Encoding 20 kDa Polypeptide of PSI Complex from Cucumber Cotyledon" Plant Cell Physiol.31. 871-879 (1990)

Y.Iwasaki、T.Sasaki 和 T.Takabe：“来自黄瓜子叶的 PSI 复合物的 20 kDa 多肽编码基因的测序和表达”植物细胞 Physiol.31。

河村一,福田正,高倍昭洋: "クロロフィル電極を用いた陽極光電流の特性" 電気学会論文誌. 109-C. 587-592 (1989)

Hajime Kawamura、Tadashi Fukuda、Akihiro Takabe：“使用叶绿素电极的阳极光电流的特性”日本电气工程师学会汇刊 109-C 587-592 (1989)。