权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Joint Study on the Function of Renin-binding Protein

肾素结合蛋白功能的联合研究

基本信息

批准号：
08044297
负责人：
FUKUI Kiyoshi
金额：
$ 1.34万
依托单位：
The Institute for Enzyme Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044297/
关键词：
renin-binding protein renin-angiotensin system endothelial cell angiotensinII gene expression renin adrenal gland

项目摘要

Renin-binding protein (RnBP) is a protein that binds to renin, forming a protein complex called high molecular weight renin. In search of its physiological function, gene cxpression of RnBP in rat aorta were examined by Northern blot hybridization. RnBP gene expression was observed in rat aorta, in addition to kidney, adrenal gland, brain and ovary. The arterial endothelial cells derived from procine aorta exhibited high level of RnBP gene expression, detected by Northern blot hybridization analysis. In order to study renin gene expression in relation to that of RnBP,porcine renin cDNA fragment was isolated by RT-PCR analysis of porcine kidney mRNA,followed by determination of the nucleotide sequence for porcine renin. Finally, the RT-PCR analysis of porcine renin mRNA in endothelial cells has shown that renin gene is also expressed in the same endothelium. RnBP gene expression was shown to be up-regulated by Angiotensin II (AII) and TGF-beta, but not by TNF-alpha in cultured endothelial cells. Therefore, reciprocal regulation of renin and RnBP by AII suggests that RnBP may participate in blood prressure regulation at vascular endothelium as a member of vascular renin-angiotensin system. We also demonstrate that an intracellular renin-angiotensin system exists in adrenal glomerulosa cells. Moreover we obtained immunocytochemical and biochemical evidence for the presence of renin within intaramitochondrial dense bodies of the zona glomerulosa.

肾素结合蛋白（Renin-binding protein，RnBP）是一种与肾素结合的蛋白质，形成一种称为高分子量肾素的蛋白质复合物。为探讨RnBP的生理功能，采用北方杂交技术检测了RnBP在大鼠主动脉中的基因表达。RnBP基因在大鼠主动脉、肾脏、肾上腺、脑和卵巢中均有表达。北方印迹杂交显示猪主动脉内皮细胞RnBP基因表达水平较高。为了研究肾素基因表达与RnBP基因表达的关系，采用RT-PCR方法从猪肾mRNA中分离出猪肾素cDNA片段，并测定其核苷酸序列。最后，对猪血管内皮细胞中肾素mRNA的RT-PCR分析表明，在同一内皮细胞中也有肾素基因的表达。在培养的内皮细胞中，RnBP基因表达被血管紧张素II（AII）和TGF-β上调，但不被TNF-α上调。因此，AII对RnBP和肾素的相互调节作用提示RnBP可能作为血管肾素-血管紧张素系统的一员参与血管内皮的血压调节。我们还表明，细胞内的肾素-血管紧张素系统存在于肾上腺肾小球细胞。此外，我们获得了免疫细胞化学和生化证据的存在下，肾素intaramitochondrial致密体的glomerulosa。