Studies on physiological functions of T cell bearing γδ TCR

我们已经揭示了一个和半千小簇，装满了一千个封闭的淋巴细胞，可以找到一个小而大的骨髓瘤。它们位于Cryptopatches（CP），可在出生后14- 17天内首次检测到。A large fraction of lymphocytes in CPs expresses c-kit，1L-7R， Thy1 and a common leukocyte antigen，LFA-1， whereas most of them remain CD3-yD1， TCRα β D1， TCRγ δ D1-yD1， slgM-yD1 and B220-yD1。population size of 1 L-2 R +在较小的智力中， CPs不包含细胞不包含抑制凋亡的非细胞携带RAG-1分子，但不包含Dendritic Stromal细胞携带CD 11 c/CD 18分子。CP中DNA复制细胞的频率高于Peyer补丁中的DNA复制频率（PPs） ... More 它比Thymic Cortex中的那个更低，而且几乎可以与Thymic Medulla中的那个相提并论。CPs的数字在老年老鼠中仍然保持相同（>114 wks），但即使在性骚扰治疗后，Thymi也在两种条件下受到关注的分享。因此，鉴于对组织的尊重，淋巴细胞组成和组织的细胞行为水平，不是PPs，孤立的淋巴细胞Folicles， Peripheral LNs，非thymus与CPs是确定的。最后， CPs在1 L-7 R的Lamina propria中的虚拟缺失--显示出在Thymic和Peripheral Lynphoid Cellularity中的原始减少。By contrast， CPs are present in germ-free mice and in athymic （nu/nu）， SCID， TCRβ×δ D1-/--D1RAG-2-D1-/-D1， PP-deficient （aly/aly）， stem cell factor （Sl/Sl-D1d-D1） and c-（W/W-D1vie D1） mutant mice。将所有这些结果结合在一起，CP是第一个识别的天然相关的Muran-L7-依赖的淋巴瘤-红细胞生成器用于T和/或B细胞的后裔可能开始在该年龄发表评论的地方。Less（低）

We have revealed that about one and a half thousand tiny clusters, filled with one thousand closely packed lymphocytes, can be found throughout the murine small and large intestinal mucosa. They are located in crypt lamina propria (cryptopatches ; CPs) and can be first detected at 14-1 7 days after birth. A large fraction of lymphocytes in CPs expresses c-kit, 1L-7R, Thy1 and a common leukocyte antigen, LFA-1, whereas most of them remain CD3ィイD1-ィエD1, TCRαβィイD1-ィエD1, TCRγδィイD1-ィエD1, slgMィイD1-ィエD1 and B220ィイD1-ィエD1. The population size of 1L-2RαィイD1+ィエD1, HASィイD1+ィエD1 and Pgp-1ィイD1+ィエD1 subsets is variable (20-50%) and the composition of CD8ィイD1+ィエD1, Ly-1ィイD1+ィエD1 and CD4ィイD1+ィエD1 subsets is smaller but also variable (3-20%). In the small intestine, CPs do not contain cells undergoing apoptosis nor cells bearing RAG-1 molecules, but do contain dendritic stromal cells bearing CD11c/CD18 molecules. The frequency of DNA replicating cells in CPs is higher than that in Peyer's patches (PPs) … More , is lower than that in the thymic cortex and is almost comparable with that in the thymic medulla. The numbers of CPs remain the same in aged mice (>114 wks) but double after estrogen treatment even though the thymi are attenuated sharply in both conditions. Thus, with respect to histogenesis, lymphocyte composition and tissue level of cellular behavior, neither PPs, isolated lymphoid follicles, peripheral LNs, nor thymus are identical with CPs. Finally, CPs are virtually absent in lamina propria of 1L-7R-deficient mice that display a profound reduction in thymic and peripheral lymphoid cellularity. By contrast, CPs are present in germ-free mice and in athymic (nu/nu), SCID, TCRβ×δ ィイD1-/-ィエD1 RAG-2ィイD1-/-ィエD1, PP-deficient (aly/aly), stem cell factor (Sl/SlィイD1dィエD1) and c-kit (W/WィイD1vィエD1) mutant mice. Taking all of these results together, CPs are the first identification of gut-associated murine lymphoid tissues where the generation of 1L-7-dependent lympho-hematopoietic progenitors for T and/or B cell descendants may start to take place at the age of commencement of weaning. Less