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Induction Mechanisms of Cholangiocarcinoma by Liver Fluke

肝吸虫诱发胆管癌的机制

基本信息

批准号：
08045064
负责人：
ANDO Katsuhiko
金额：
$ 4.61万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08045064/
关键词：
Opisthorchis viverrini cholangiocarcinoma epidemiology animal experiment

项目摘要

Infection rate of Opisthorchis viverrini (Ov) in snail and fish were surveyed, at 2 lakes located in Northeast Thailand. No Ov cercariae were detected in snails. Whereas Ov metacercariae were detected in cyprinoid fish caught at both lakes.These metacercariae were given to hamster to induce cholangiocell carcinomas.Chemical carcinogen (DMN) induced cholangiocell carcinomas in 30% of the animals at 23 weeks after the termination of the treatment. An infection with Ov promoted the carcinogenesis to 100%. The preimmunization of the animals by crude extracts of Ov influenced the cancer development variously, regulating and progressing. Ov infection alone resulted in a variety of pathological changes such as dilatation of bile duct, bile ductule prolifertation, and liver cirrhosis but not cancer. Oncofetal type of extracellular matrix tenascin was examined by immunohistochemistry. The positive staining was found in connective tissues surrounding the dilatated ducts and the stroma of cancers, and strongly in the stroma flame of necrotic cancer nodules.Nineteen Thai cases of cholangiocellular carcinoma (CCC) and 23 Japanese cases of CCC were compared for clinicopathological and biological features. A review of histological type revealed poorly differentiated and mucinous adenocarcinoma to be somewhat more prevalent in the Thai cases, and peribiliary fibrosis and adenomatous hyperplasia were found to be much more frequent in the Thai cases in noncancerous area of the liver. No significant differences in expression of p53 protein or c-erbB.2 protein were found between the two series, but proliferative activity evaluated by mean MIB1 L.I.was significantly higher in the Thai cases.Nucleotide sequence of ITS2 in Ov ribosomal DNA was determined. They were compared among Ov taken from three districts (Chonnabot, Chaiyaphum and Kalasin) in northeast Thailand but there was no difference among them.

在泰国东北部的两个湖泊调查了钉螺和鱼类的猫尾后睾吸虫（Ov）感染率。钉螺体内未检出Ov尾蚴。用化学致癌物（DMN）诱发仓鼠胆管癌，23周后有30%的仓鼠发生胆管癌，而在两个湖的鲤科鱼类中均检出Ov囊蚴。Ov感染可促进100%的癌变。Ov粗提物预免疫动物对肿瘤的发生、发展有不同程度的影响。Ov感染可引起胆管扩张、胆管增生、肝硬化等多种病理改变，但不引起肝癌。免疫组化检测癌胚型细胞外基质腱生蛋白。阳性染色见于胆管周围结缔组织和癌间质，在坏死癌结节间质火焰中染色较强。比较了19例泰国胆管细胞癌（CCC）和23例日本CCC的临床病理和生物学特征。对组织学类型的回顾显示，泰国病例中低分化和粘液腺癌更为常见，并且发现泰国病例中肝脏非癌区域的胆管周围纤维化和腺瘤样增生更为常见。两个系列的p53蛋白或c-erbB.2蛋白表达无明显差异，但泰国病例的平均MIB 1 LI评价的增殖活性明显较高。确定了Ov核糖体DNA中ITS 2的核苷酸序列。他们之间的Ov采取的三个地区（Chonnabot，Chaiyaphum和卡拉辛）在泰国东北部进行了比较，但没有差异。