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E.coli Genome Project

大肠杆菌基因组计划

基本信息

批准号：
08309009
负责人：
HORIUCHI Takashi
金额：
$ 7.04万
依托单位：
(National Institute For Basic Biology) Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08309009/
关键词：
Escherichia coli genome analysis sequencing genome project データパンク WWW 塩基配列決定データバンク

项目摘要

Escherichia coli has been playing significant roles as a model organism in establishment of a number of basic concepts in molecular biology, and the huge accumulation of data has contributed to a better understanding of cellular processes of E.coli and numerous other organisms. Such being the case, completion of analysis of the E.coli genome is vital as a reference for structures and functions of genes in various organisms.The E.coli genome project in Japan was begun in 1989, the objective being to analyze the sequence of the E.coli genome from 0 minutes and in a clockwise direction. In March 1995, the DNA sequences of the 0-13 min region on the genetic map had been determined. In April 1995, we set up a new group consisting of 12 Japanese researchers to analyze the DNA sequence of the region from 13 to 69 minutes, using a new protocol. We separated the sequencing protocol into four steps ; 1) Fragment preparation, 2) Template preparation, 3) Sequencing and 4) Analysis and storage of data. Each member of the group is responsible for one or two of these steps.The sequence of the 13-69 min region was completed in January 1997 and the E.coli genome in a contiguous sequence was completed by combining our sequence data with the sequence data of the 69-100 min region reported by Blattner's group. Analysis of our sequences was published in four separate papers, registered in DDBJ and can be found on the Web. Sequence analysis of the entire E.coli genome is under way.

大肠杆菌作为一种模式生物在分子生物学的许多基本概念的建立中发挥着重要作用，大量数据的积累有助于更好地理解大肠杆菌和许多其他生物的细胞过程。因此，完成大肠杆菌基因组的解析，作为各种生物体的基因结构和功能的参照，是非常重要的。日本的大肠杆菌基因组计划始于1989年，目的是解析从0分钟开始的顺时针方向的大肠杆菌基因组序列。1995年3月，确定了遗传图谱上0-13 min区域的DNA序列。1995年4月，我们成立了一个由12名日本研究人员组成的新小组，使用一种新的方案分析了13至69分钟区域的DNA序列。我们将测序方案分为四个步骤：1）片段制备，2）模板制备，3）测序和4）数据分析和存储。1997年1月完成了13-69 min区域的序列测定，并将我们的序列数据与Blattner小组报告的69-100 min区域的序列数据相结合，完成了大肠杆菌基因组的连续序列测定。我们的序列分析发表在四篇独立的论文中，在DDBJ注册，可以在网上找到。整个大肠杆菌基因组的序列分析正在进行中。