STUDY ON DETERIORATING MECHANISM OF PERITONEAL FUNCTIONUSING CULTURED MESOTHELIAL CELL

培养间皮细胞对腹膜功能恶化机制的研究

• 批准号: 07650967
• 负责人: HORIUCHI Takashi
• 金额: $ 1.34万
• 依托单位: UNIVERSITY OF EAST ASIA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07650967/
• 关键词: peritoneal dialysis; peritoneal fibrosis; peritoneal mesothelial cell; DNA synthesis; glucose; albumin; membrane permeability; chemical fibrosis

Incidence of sclerosing encapsulating peritonitis (SEP) increases as a duration of peritoneal dialysis is prolonged. It has been hypothesized that hydrated degeneration of collagen matrix is possibly occurred at high concentration of glucose and it may result in reducing cell growth in the mesothelium.To understand both mechanism of regeneration of the mesothelial cell under high permeable state and change in peritoneal permeability under chemically induced peritonitis, in vitro experiments with cultured mesothelial cells and in vivo experiments with intraperitoneal injection of Chlorohexidine gluconate (CHG) were carried out. After one to five week exposure of type I collagen coated multi-titer plate. to varying concentrations of glucose, growth of mesothelial cells seeded were suppressed at 2g/dl of albumin supplement no matter what concentration of glucose supplemented was. Without high concentration of albumin, DNA synthesis was in a dose-dependent manner of glucose. There was no significant difference of DNA synthesis between groups with and without one-week glucose immerse when mesothelial cells were cultured only 10%FCS/MEM.20ml/kg of 0.08-0.2wt% CHG was injected into the abdominal space of male Wistar rat (at 8 weeks). Eight out of 11 rats were survived and 5 out of 8 rats developed adhesions and one developed SEP-like degeneration. Although there was no statistically signifficant change, MTAC increased from 1.18(]SY.+-。[)0.66ml/min to 1.92(]SY.+-。[)0.6ml/min in the first three weeks (p=0.09) and slightly decreased at the 4th week.

随着腹膜透析时间的延长，硬化性包膜性腹膜炎（SEP）的发生率增加。据推测，在高浓度葡萄糖的作用下，胶原基质的水合变性可能发生，并可能导致间皮细胞生长减少。为了解化学诱导腹膜炎高通透状态下间皮细胞的再生机制和腹膜通透性的变化，采用体外培养间皮细胞实验和体内注射葡萄糖酸氯己定（CHG）实验。I型胶原包被多效价板暴露一至五周后。对于不同浓度的葡萄糖，无论葡萄糖浓度如何，添加2g/dl白蛋白都抑制了间皮细胞的生长。在没有高浓度白蛋白的情况下，DNA合成呈葡萄糖剂量依赖性。当间皮细胞仅培养10%FCS/MEM时，葡萄糖浸泡1周组与未浸泡1周组间DNA合成无显著差异。雄性Wistar大鼠腹腔注射0.08 ~ 0.2wt% CHG 20ml/kg。11只大鼠中8只存活，8只大鼠中5只发生粘连，1只发生sep样变性。虽然没有统计学意义的变化，但MTAC在前三周从1.18(]SY.+- [)0.66ml/min上升到1.92(]SY.+- [)0.6ml/min (p=0.09)，第4周略有下降。

项目成果

Hayashi K,Horiuchi T,et al: "Changes in peritoneal permeability due to chemical fibrosis." Advances in Peritoneal Dialysis 13. (in press). (1997)

Hayashi K、Horiuchi T 等人：“化学纤维化引起的腹膜通透性变化。”

Horiuchi T,Nanba J,et al: "Suppression of DNA syhthesis of cultured mesothelial cells by supplemented albumin." Int J Artif Organs 20. (in press). (1997)

Horiuchi T、Nanba J 等人：“补充白蛋白对培养间皮细胞 DNA 合成的抑制”。

Hayashi K,Horiuchi T,Ohta Y,Kumano K: "Changes in peritoneal permeability due to chemical fibrosis" Advances in Peritoneal Dialysis. 13(in press). (1997)

Hayashi K、Horiuchi T、Ohta Y、Kumano K：“化学纤维化引起的腹膜通透性变化”腹膜透析进展。