New diagnostic methods of acute rejection after living donor liver transplantation

活体肝移植术后急性排斥反应诊断新方法

• 批准号: 08407036
• 负责人: MAKUUCHI Masatoshi
• 金额: $ 20.61万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08407036/
• 关键词: Liver Transplantation; Acute rejection; Cytokines; Eosinophilia; soluble II L-2 recepter; IL-6; TNF

At Tokyo University Hospital, 63 living-related liver transplantation have been performed since January, 1996. There were 27 males and 36 females (Average age : 14.9 years, range : 8 months-62 years). The diagnoses were biliary atresia in 40, primary biliary cirrhosis in 8, liver cirrhosis in 7, Alagille syndrome in 2 and others in the remaining 5. FK506 and steroid were employed as main immunosuppressive drugs. Acute rejection was diagnosed by the elevation of T-Bil, GOT, GPT, ALP and G-GTP, which was confirmed histologically. 15 of 52 patients showed acute rejection, whereas the remaining 37 had no rejection. Histology showed cellular infiltration in the Gulissonian triad, destruction of the bile duct and endothelialitis. Furthermore, liver dysfunction with eosinophilia in the peripheral blood highly suggested the presence of acute rejection. Whereas alteartions of cytokines in the peripheral blood were not specific to the acute rejection.

自1996年1月以来，东京大学医院共进行了63例活体肝移植。男27例，女36例，年龄8个月~62岁，平均14.9岁。诊断为胆道闭锁40例，原发性胆汁性肝硬变8例，肝硬变7例，Alagille综合征2例，其他5例。T-Bil、GOT、GPT、ALP、G-GTP升高诊断为急性排斥反应，并经组织学证实。52例中15例出现急性排斥反应，37例无排斥反应。组织学检查显示古里森三联症的细胞浸润、胆管破坏和内皮炎。此外，肝功能障碍伴外周血嗜酸性粒细胞增多高度提示急性排斥反应的存在。而外周血中细胞因子的变化并不是急性排斥反应的特异性。

项目成果

Harihara Y, Makuuchi M, Kawasaki H, et al.: "Initial experience with Living-related liver transplantation at the University of Tokyo"Transplantation Procceedings.. 30. 129-131 (1998)

Harihara Y、Makuuchi M、Kawasaki H 等：“东京大学与生活相关的肝移植的初步经验”移植论文集.. 30. 129-131 (1998)

針原康, 幕内雅敏: "わが国における肝移植の現状" Medical Practice. 13. 1417-1419 (1996)

Yasushi Harihara、Masatoshi Makuuchi：“日本肝移植的现状”医疗实践 13. 1417-1419 (1996)。

Kita Y, Uchida S, Makuuchi M: "New Methods to Detect Donor-Type DNA in HLA-DRB1-Matched Living-Relates Liver Transplant Recipients." Transplantation Procceedings.30. 3493-3495 (1998)

Kita Y、Uchida S、Makuuchi M：“检测 HLA-DRB1 匹配的生活相关肝移植受者中供体型 DNA 的新方法。”

Harihara Y,Makuuchi M,.et al.: "Difference in immunosuppressive effects of antibodies to ICAM-1 and LFA-1 between hepatic and cardiac allografts"Transplantation Procceedings. 28. 1368-1369 (1996)

Harihara Y、Makuuchi M 等人：“肝脏和心脏同种异体移植物之间 ICAM-1 和 LFA-1 抗体的免疫抑制作用差异”移植论文集。

針原康、幕内雅敏: "生体部分肝移植 Living-related partial Liver Tranplantation"日本内科学会雑誌. 86. 1055-1061 (1997)

Yasushi Harihara、Masatoshi Makuuchi：“与活体相关的部分肝脏移植”日本内科学会杂志 86. 1055-1061 (1997)。