Xenogeneic per fusion experiment and treatment for patients with liver failure using a non woven fabric bioreactor.

使用无纺布生物反应器对肝衰竭患者进行异种灌注融合实验和治疗。

• 批准号: 11357010
• 负责人: MAKUUCHI Masatoshi
• 金额: $ 24.45万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11357010/
• 关键词: Hybrid bioartificial liver; A non woven fabric bioreactor; porcine hepatocyte; Direct hemoperfusion treatment; ハイブリット型人工肝臓; ハイブリッド型人工肝臓; 全血直接潅流治療; 異種潅流治療; 人工肝臓; 異種全血直接灌流治療; ハイブリッド型人工肝臓システム

We developed a new system of xenogeneic direct hemoperfusion of bioartificial liver support consisting of a leukocyte adsorbent, an immunoglobulin adsorbent, and a substitute unit for hepatic function, that is, whole liver or bioreactor. A further improvement of our system by incorporating a leukocytapheresis (LCAP) device to return the leukocyte-rich portion of the per fused blood back to the patient's body directly was undergone. Influx of porcine endogenous retrovirus (PERV) into the human body has been a controvertial subject. We developed resusciteting separated plasma per fusion method, which are promising in that the patient's circuit and the bioartificial liver circuit are separated by a semipermiable membrane, which can prevent any kind of virus from flowing into the patient's circuit.

我们开发了一种新的生物人工肝支持异种直接血液灌流系统，该系统由白细胞吸附剂、免疫球蛋白吸附剂和肝功能替代单元（即全肝或生物反应器）组成。我们对系统进行了进一步改进，加入了白细胞分离术 (LCAP) 装置，将灌注血液中富含白细胞的部分直接返回患者体内。猪内源性逆转录病毒（PERV）流入人体一直是一个有争议的话题。我们开发了复苏分离血浆融合法，这种方法很有前途，因为患者的回路和生物人工肝回路被半透膜隔开，可以防止任何种类的病毒流入患者的回路。

项目成果

Katsutoshi Naruse, Masatoshi Makuuchi: "Development of bioartificial liver"Igaku no Ayuimi. 195. 991 (2000)

成濑胜俊、幕内正俊：《生物人工肝的开发》医学之鲇见。

Katsutoshi Naruse: "Development and Perspctives of bioantificial liver support"Hepato-pastroenterlogy. 49. 91-95 (2002)

Katsutoshi Naruse：“生物抗肝支持的发展和前景”肝病学。

成瀬勝俊: "人工肝臓と肝細胞培養"Annual Review 消化器2000. 51-57 (2000)

Katsutoshi Naruse：“人工肝脏和肝细胞培养”年度评论胃肠病学 2000. 51-57 (2000)

Katsutoshi Naruse, Masatoshi Makuuchi: "Bioartificial liver and hepatocyte culture"Annual review Syoukaki (Tyugai Igakusya). 51-57 (2000)

Katsutoshi Naruse、Masatoshi Makuuchi：“生物人工肝和肝细胞培养”年度评论Syoukaki（Tyugai Igakusya）。

成瀬勝俊, 酒井康行: "再生医学における肝細胞の分離・再構築法"Surgery Frontier. 8(3). 69-75 (2001)

Katsutoshi Naruse，Yasuyuki Sakai：“再生医学中肝细胞的分离和重建方法”Surgery Frontier 8(3) 69-75 (2001)。