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Phylogenetic relationship of wild Petunia taxa by RFLP analysis of chloroplast DNA

通过叶绿体 DNA RFLP 分析野生矮牵牛类群的系统发育关系

基本信息

批准号：
08456016
负责人：
ANDO Toshio
金额：
$ 3.26万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456016/
关键词：
Petunia Calibrachoa RFLP Solanaceae chloroplast DNA molecular phylogeny

项目摘要

Phylogenetic relationship among the species of Petunia Juss. was analyzed by restriction fragment length polymorphism (RFLP) of chloroplast DNA digested by 20 restriction enzymes and hybridized with 12 cloned Nicotiana cpDNA fragments as probes. A total of 89 useful RFLPs were detected and one Wagner parsimonious tree was obtained using those data. In the tree, both Petunia sensu Wijsman and Calibrachoa were monophyletic but they showed paraphyletic relationship relative to Nicotiana langsdorffii. Six Petunia Juss. species that are not assigned to Calibrachoa were included in the monophyletic clade composed of all species of Calibrachoa used in this study. Calibrachoa parviflora and C.pygmaea, previously thought to be different from the other species of Calibrachoa, formed a clade within the genus Calibrachoa. Among other Calibrachoa species, one geographical clade emerged that consists of the species found in Uruguay and southern Rio Grande do Sul state in Brazil. The clades of Petunia sensu Wijsman obtained from the RFLP data could not be interpreted as groups that correspond with traditional classification. However, four small clades were formed in which the embraced species had some common characters, such as cross-compatibility, floral morphology, and geographical distribution.

矮牵牛物种之间的系统发育关系。通过叶绿体 DNA 的限制性片段长度多态性 (RFLP) 进行分析，叶绿体 DNA 经 20 种限制性内切酶消化，并与作为探针的 12 个克隆烟草 cpDNA 片段杂交。总共检测到 89 个有用的 RFLP，并使用这些数据获得了一棵瓦格纳简约树。在树中，Petunia sensu Wijsman 和 Calibrachoa 都是单系的，但它们与 Nicotiana langsdorffii 表现出并系关系。六个矮牵牛朱斯。未归入 Calibrachoa 的物种均包含在由本研究中使用的所有 Calibrachoa 物种组成的单系支中。 Calibrachoa parviflora 和 C.pygmaea 以前被认为与 Calibrachoa 的其他物种不同，但它们在 Calibrachoa 属内形成了一个分支。在其他 Calibrachoa 物种中，出现了一个地理分支，由乌拉圭和巴西南里奥格兰德州南部发现的物种组成。从 RFLP 数据中获得的 Petunia sensu Wijsman 的进化枝不能解释为与传统分类相对应的群体。然而，形成了四个小分支，其中包含的物种具有一些共同特征，例如交叉兼容性、花形态和地理分布。