Phylogenetic relationship of wild Petunia taxa by RFLP analysis of chloroplast DNA

标题矮牵牛种间的系统发育关系。用20种酶切后的叶绿体DNA限制性内切片段长度多态性（RFLP）与12个克隆的烟草cpDNA片段杂交作为探针进行分析。共检测到89个有用的RFLPs，并利用这些数据获得了一个Wagner简约树。矮牵牛花（Petunia senssuwijsman）和小叶牵牛花（calibrhoa）均为单系，但与烟叶（Nicotiana langsdorffii）呈副系关系。六个佩妮·杰斯。本研究中所使用的单系分支包括了未归属于足甲亚的物种。以前被认为不同于其他种类的Calibrachoa parviflora和C.pygmaea在Calibrachoa属中形成了一个分支。在其他的Calibrachoa物种中，出现了一个地理分支，由在乌拉圭和巴西南巴西格兰德州南部发现的物种组成。从RFLP数据得到的矮牵牛枝不能被解释为与传统分类相对应的类群。然而，在四个小分支中，被拥抱的物种具有一些共同的特征，如交叉相容性、花形态和地理分布。

Phylogenetic relationship among the species of Petunia Juss. was analyzed by restriction fragment length polymorphism (RFLP) of chloroplast DNA digested by 20 restriction enzymes and hybridized with 12 cloned Nicotiana cpDNA fragments as probes. A total of 89 useful RFLPs were detected and one Wagner parsimonious tree was obtained using those data. In the tree, both Petunia sensu Wijsman and Calibrachoa were monophyletic but they showed paraphyletic relationship relative to Nicotiana langsdorffii. Six Petunia Juss. species that are not assigned to Calibrachoa were included in the monophyletic clade composed of all species of Calibrachoa used in this study. Calibrachoa parviflora and C.pygmaea, previously thought to be different from the other species of Calibrachoa, formed a clade within the genus Calibrachoa. Among other Calibrachoa species, one geographical clade emerged that consists of the species found in Uruguay and southern Rio Grande do Sul state in Brazil. The clades of Petunia sensu Wijsman obtained from the RFLP data could not be interpreted as groups that correspond with traditional classification. However, four small clades were formed in which the embraced species had some common characters, such as cross-compatibility, floral morphology, and geographical distribution.