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Study of Solanaceous plants from eastern continental temperate climate : collection, evaluation, and molecular phylogeny

东部大陆温带气候茄科植物的研究：收集、评价和分子系统发育

基本信息

批准号：
14405023
负责人：
ANDO Toshio
金额：
$ 8.7万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14405023/
关键词：
Molecular phylogeny Gene resource evaluation Solanaceae International research cooperation Argentina

项目摘要

We conducted three expeditions to Argentina to collect Solanaceous plants. In total, 224 accessions were collected, of which 126 were collected as seeds. We sowed 74 accessions, 34 germinated, and 30 reached blooming. We idendified 20 accessions that had ornamental values. All had enough heat and cold tolerance, and thought to be a good breeding materials. Among them, Lycium, Nicotiana, Nierembergia, Solanum were significant genera.We also conducted phylogenetic study on 24 genera, 35 species plus two outgroups using DNA that was collected during the expeditions. Two intergenic spacers (rpl16-rpl14 and trnT-L-L-F) and an intron (trnK) sequences of chloroplast DNA were determined and phylogenetic trees were calculated from aligned sequences. Argentine Solanaceous plants consisted of five clades that are equivalent to sub families, Schwenckioideae, Cestroideae, Nicotianoideae, Solanoideae, and Petunioideae in addition with a combined clade (Nicotianoideae+Solanoideae) that have been called the "X=12 clade." Sclerophylax was placed outside of Solanaceae+Convolvulaceae clade. Two Patagonian endemic genera, Benthamiella and Pantacantha formed a clade that belonged to Cestroideae. Petunioideae was divided into two subclades, one consists of Bouchetia, Leptoglossis, and Nierembergia and another of Calibrachoa, Fabiana, and Petunia. Petunia patagonica was included in a clade of Fabiana species.

我们对阿根廷进行了三次考察，采集茄科植物。共收集材料224份，其中种子126份。我们播种了74株，34株发芽，30株开花。我们确定了20件具有观赏价值的藏品。均具有足够的耐热性和耐寒性，被认为是优良的育种材料。其中枸杞属（Lycium）、烟叶属（Nicotiana）、尼伦堡属（nieembergia）、茄属（Solanum）为显著属。我们还利用考察期间收集的DNA对24属、35种和2个外群进行了系统发育研究。测定了叶绿体DNA的两个基因间间隔序列（rpl16-rpl14和trnT-L-L-F）和一个内含子（trnK）序列，并根据比对序列计算了系统发育树。阿根廷茄科植物包括五个分支，相当于亚科，Schwenckioideae, Cestroideae, Nicotianoideae， Solanoideae和Petunioideae，此外还有一个组合分支（Nicotianoideae+Solanoideae），被称为“X=12分支”。硬叶位于茄科+旋花科分支的外侧。两个巴塔哥尼亚特有属，Benthamiella和Pantacantha形成了一个分支，属于Cestroideae。矮牵牛总科分为两个亚支，一个由Bouchetia、Leptoglossis和Nierembergia组成，另一个由Calibrachoa、Fabiana和Petunia组成。巴塔哥尼卡矮牵牛花被列入费比亚纳属的一个分支。