Biosynthetic Studies of terpenoids produced by actinomycetes

放线菌产生的萜类化合物的生物合成研究

基本信息

批准号：
08456057
负责人：
SETO Haruo
金额：
$ 4.35万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

It has long been believed that isopentenyl diphosphate (IPP), a starting unit of terpenoid Compound, is produced by the classical mevalonate pathway. Recent finding of the new non- mevalonate pathway by Rohmer raised a question which of the two pathways do Streptomycetes utilize? In order to address this interesting question, we have studied the biosynthesis of terpenoidal compounds produced by Streptomyces such as naphterpin, longestin, carquinostatin and novobiocin. In addition, the biosynthesis of a terpenoid BE-40644 produced by Actionoplanes was also studied byusing ^<13>C- and ^2H-labeled precursors.AS a result, it has turned out that several Streptomycetes (longestin, carquinostatin and novobiocin producers) possess only the non-mevalonate pathway, while some others (naphterpin, furaquinocin, napyradiomycin and BE-40644producers) can utilize both the pathways. Streptomycetes belonging to the latter group use the non-mevalonate pathway at earlier growth stage and then gradually the pathway is switched to the mevalonate pathway. Such switching of the metabolic pathways depending on the growth has never been reported.Then we purified HMG Co-A reductase from Streptomyces aureouvifer, a producer of naphterpin, and prepared a DNA probe based on the obtained amino acid sequence. Southern hybridization analysis showed that there seems to be no relationship between the presence of the mevalonate pathway and the taxonomical position of Streptomyces organisms.

长期以来，人们一直认为异戊烯基二磷酸（IPP）是萜类化合物的起始单位，是由经典的甲戊酸途径产生的。罗默（Rohmer）对新的非大甲酸途径途径的最新发现提出了一个问题，链霉菌使用了两种途径中的哪一种？为了解决这个有趣的问题，我们研究了由链霉素，longestin，carquinostatin和Novobiocin产生的萜类化合物的生物合成。此外，还通过使用 ^<13> c-和 ^2H标记的前体研究了由动作联合产生的萜类BE-40644的生物合成。结果，结果证明，几个链霉菌（多种链霉菌）（longestin，carquinostatin and novobiocin）仅具有其他途径，而其他人则具有其他途径，而其他链球菌则（小说）（小说） Furaquinocin，Napyradiomycin和Be-40644Producers）可以利用这两种途径。属于后一组的链霉菌在较早的生长阶段使用非甲状腺素途径，然后逐渐将途径切换到甲谷酸酯途径。根据生长的情况，这种代谢途径的这种切换从未得到报道。南部杂交分析表明，甲谷酸酯途径的存在与链霉菌生物的分类学位置之间似乎没有关系。