权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on the inshore migration mechanism in the Japanese eel inferred by mtDNA and otolith microstructure analyzes

通过mtDNA和耳石微结构分析推断日本鳗鱼近海洄游机制的研究

基本信息

批准号：
08456094
负责人：
TUKAMOTO Katsumi
金额：
$ 4.99万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456094/
关键词：
eel inshore migration mtDNA control region population analysis oltolith daily ring metamorphose 調節領域

项目摘要

Mechanism of inshore migration of the Japanese eel was studied as follows :1. Age determination and estimation of migratory historyAge at recruitment of the glass eel to East Asian coasts was 143-206 days after hatching. It was revealed that the younger individuals with the higher growth rate migrated to rivers at the lower latitudes. Analysis of otolith Sr/Ca ratio by EPMA showed that the leptocephali would begin to metamorphose at mean age of 130 d (range : 80-160 d) and it last for about 30 d (20-40 d).2. Population structureComparing 500bp sequences of 16SrRNA cording region on mtDNA enabled to identify all 18 species of genus Anguilla, even if the samples would be eggs or small leptocephali which could not be identified with morphological features. A part of the mitochomdrial control region of 47 Anguilla japonica collected from Ibaragi, Kanagawa and Kagoshima prefecture in Japan were determined and showed that all individuals belong to one reproductive population. Furthre analysis including the previously reported 31 sequence data of A.japonica collected from Taiwan, Korea and China suggested a single mating population for this species. Thus, stock management of A.japonica should be performed not only within Japan but among all the East Asian countries.

本研究对日本鳗鲡的近岸洄游机理进行了如下研究：1.日本鳗鲡的年龄测定和洄游史估算日本鳗鲡在东亚沿海的招募年龄为孵化后143-206天。结果表明，具有较高生长速度的年轻个体迁移到低纬度的河流。电子探针分析耳石Sr/Ca比值表明，细头蚴在平均130 d（80-160 d）开始开始变质，变质持续时间约30 d（20-40 d）.群体结构比较mtDNA上16 SrRNA编码区500 bp序列，即使样品是不能用形态特征鉴定的卵或小的钩端螺旋体，也能鉴定出安圭拉属的所有18个种。对日本茨城县、神奈川县和鹿儿岛县采集的47只日本鳗鲡（Anguillajaponica）的部分线粒体控制区进行了测定，结果表明，所有个体都属于同一个生殖群体。进一步分析了31个来自台湾、韩国和中国的日本金龟子的序列数据，结果表明该种为单一交配型种群。因此，不仅在日本国内，而且在所有东亚国家之间都应该进行日本蒿的库存管理。