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Histological studies on differntiation and systematization of vitamin A-storing cells in vertebrates.

脊椎动物维生素 A 储存细胞分化和系统化的组织学研究。

基本信息

批准号：
08457002
负责人：
WAKE Kenjiro
金额：
$ 5.57万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457002/
关键词：
Vitamin A Vitamin A-strong cell Hepatic stellate cell Collagen Intralobular heterogeneity kidney Lamprey Mesangial cell メタンギウム細胞コラーゲン形成肝臓星細胞腸ブタ

项目摘要

In order to know the heterogeneity of stored vitamin A in the hepatic stellate cells, we examined pig liver by the use of gold chloride method. Vitamin A-lipid droplet morphometry was performed under oil immersion and the calculated volumes were plotted on computerized maps. The hepatic lobule was sub divided into 5-zone/5-region comparmentalization. Distribution of values exhibited a district zonal gradient, higher at peripheral zones 1 and 2, dcreased over intermediate zone 3 toward centrilobular zones 4 and 5. Within a single zone, variations were obvious, forming a regional gradient ; higher at periportal than mideptal regions. Along the periphery, inlet venules of portal veins were quantified, revealing an occurrence rate of 60% at periportal, and 5% at midesptal regions.Abundant vitamin A was found in the mesangial cells in the lamprey kidney. We discussed similarities of the this cell type with the hepatic stellate cell. From our both present and previous findings on the lamprey, we concluded that vitamin A is stored in various splanchnic organs but not in the somatic organs. In the mammalian species, vitamin A is stored only in the liver, however, after the administration of excess vitamin A the fibroblastic cells in the splanchnic organs incorporated and stored it in the cytopalsm. Vitamin A-storing cell system is a system of mesenchymal cell system existing only in the splanchnic organs of vertebrates and vitamin A-storing cells reveal heterogeneity not only among splanchnic organs but also within a single organ.

为了解肝星状细胞中维生素A的储存异质性，我们用氯化金方法对猪肝进行了检测。在油浸条件下进行维生素A-脂滴形态测量，并将计算的体积绘制在计算机地图上。将肝小叶分为5区/5区对照。值的分布呈区域带状梯度，外周1区和2区较高，中间3区向小叶中央4区和5区逐渐减小。在单个区域内，变化明显，形成区域梯度，门周高于中部。门静脉入口小静脉在门静脉周围的出现率为60%，在门静脉中段的出现率为5%。七鳃鳗肾脏的系膜细胞中含有丰富的维生素A。我们讨论了该细胞类型与肝星状细胞的相似性。根据我们目前和以前对七鳃鳗的研究结果，我们得出结论，维生素A储存在各种内脏器官中，而不是在身体器官中。在哺乳动物中，维生素A只储存在肝脏中，然而，在摄入过量的维生素A后，内脏器官中的成纤维细胞将其结合并储存在细胞质中。维生素A储存细胞系统是仅存在于脊椎动物内脏器官中的间充质细胞系统，维生素A储存细胞不仅在内脏器官之间表现出异质性，而且在单个器官内也表现出异质性。