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Studies on movement of sinusoidal cells in the liver

肝窦细胞运动的研究

基本信息

批准号：
05454134
负责人：
WAKE Kenjiro
金额：
$ 5.95万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Morphology of the hepatic stellate cells isolated from the rat liver was observed phase-contrast microscorpy with or without Video-recording. Cultured stellate cells change their forms continuously. After seeding on plastic, stellate cells extend thin fan-shaped membranous processes around the nucleus. As these membranous processes expand further, the processes change to dendritic in appearance. Two days after seeding the cells change their shapes gradually from dendritic to membranous. When kupffer cell-conditioned medium (KCCM) is added to stellate cells two days after seeding, the membranous processes change dramatically to dendritic. To know the potential mediators stimulating the formation of dendritic processes in KCCM,PGE_2 was added to the primary culture. Similar changes in stellate cell morphology occured rapidly after adding PGE_2 (Wake, K.et al.1993).Three-dimensional structure of the sinusoidal wall and the space of Disse in the liver were examined by the computer-assisted reconstruction of serial sections of the Golgi stained preparations, transmission and scanning electron microscopy. From the anatomical point of view, contraction and relaxation of the stellate cells or certain pressure loaded upon the endothelial cells can accelerate fluid-exchange between the blood stream and the space of Disse through the endothelial fenestrations (Wake, K.1995).In order to explore the mode of adhesion of lymphocytes and monocytes to endothelial cells we observed the sinusoid in vivo under a microscope equipped with a Video-camera. After injection of carbon tetrachloride, numerous mononuclear cells sticked to the endothelial cells and some of them showed ameboid movement. Within the granuloma formations only a mechanical wall, but a dynamic cell-complex.

用相差显微镜观察大鼠肝星状细胞的形态学变化。培养的星状细胞不断地改变它们的形态。在塑料上接种后，星状细胞在细胞核周围延伸出薄的扇形膜状突起。当这些膜状突起进一步扩张时，突起在外观上变为树枝状。接种后两天，细胞逐渐从树突状变为膜状。当库普弗细胞条件培养基（KCCM）加入到星状细胞接种后两天，膜突起显着变化为树突状。为了解促进KCCM树突状突起形成的可能介质，在原代培养中加入PGE_2。加入PGE_2后，星状细胞的形态也迅速发生类似的变化（Wake，K.et al.1993）。通过计算机辅助重建Golgi染色标本的连续切片、透射和扫描电镜，观察了肝窦壁和Disse间隙的三维结构。从解剖学的角度来看，星状细胞的收缩和舒张或施加在内皮细胞上的一定压力可以通过内皮开窗加速血流与Disse间隙之间的液体交换（Wake，K.1995）。为了探索淋巴细胞和单核细胞与内皮细胞的粘附模式，我们在配有摄像机的显微镜下观察了体内窦状隙。注射四氯化碳后，大量单个核细胞贴附于内皮细胞上，部分细胞呈阿米巴样运动。在肉芽肿内形成的只有一个机械壁，但一个动态的细胞复合体。