Expression of new cell-cell adhesion molecule and mucin genes in cancers : its relationship with invasion or metastasis of cancers

新细胞间粘附分子和粘蛋白基因在癌症中的表达：与癌症侵袭或转移的关系

• 批准号: 08457064
• 负责人: YONEZAWA Suguru
• 金额: $ 5.18万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457064/
• 关键词: Thrombomodulin; Squamous cell carcinoma; Adenocarcinoma; MUC1 mucin; MUC2 mucin; MUC5AC mucin; Sialic acid; Prognosis of patient carrying cancer; ムチン; 食道癌; 消化器癌; in situ hybridization; 肺癌; ムチン抗原

1. Squamous cell carcinoma(1) Esophageal squamous cell carcinoma (S CC) cell lines produced the same thrombomodulin (TM) molecule as human endothelial cell line. The TM-low-expressing esophageal SCC cells expressed high invasiveness than TM-high-expressing esophageal SCC cells.(2) In SCCs of oral cavity and lung, the tumor cells that were positive for TM expression were significantly rarer in the lymph node metastatic lesions than in the primary tumors.(3) In SCCs of pharynx and larynx, and of esophagus, sialylated MUC1 mucin (DF3 antigen) was an effective tumor marker. The expression of sialyl oligosaccharides of DF3 antigen was related to poor prognosis in esophageal SCCs.2. Adenocarcinoma(1) Even in the expansive growth type tumors of pancreas and intrahepatic bile duct, invasive growth areas of the tumors acquire a characteristic of MUC1 mucin expression that is usually seen in the invasive growth type tumors.(2) In situ hybridization study disclosed that MUC2 mucin expression in m … More ost of expansive growth type tumors of pancreas and intrahepatic bile duct was regulated by MUC2mRNA.Patients with positive MUC2mRNA expression in the tumors showed significantly better survival than those with negative MUC2mRNA expression in the tumors.(3) Patterns of MUC5AC (gastric type) and MUC2 (intestinal type) gene expression in "mucin producing tumors of the pancreas (MPTs)", which usually reveal less invasive growth, showed variety among the subtypes of MPTs.(4) In the tumors of pancreas and intrahepatic bile duct, sialic acid epitope of MUC1 mucin seems to play an important role in the invasion growth.(5) In carcinomas of ampulla of Vater and stomach, the patients with MUC1(+) expression showed poorer outcome than those with MUC1(-) expression, whereas the patients with MUC2(+) expression showed more favorable outcome than those with MUC2(-) expression.(6) Significant reduction of pericryptal fibroblast sheath in colorectal epithelial neoplasms reflects progression in the adenoma-carcinoma sequence. Less

1.鳞状细胞癌（1）食管鳞状细胞癌（S CC）细胞系产生与人内皮细胞系相同的血栓调节蛋白（TM）分子。TM低表达的食管鳞癌细胞比TM高表达的食管鳞癌细胞表达更高的侵袭性。(2)在口腔和肺鳞癌中，TM表达阳性的肿瘤细胞在淋巴结转移灶中明显少于原发灶。(3)在咽喉和食管的SCC中，唾液酸化MUC 1粘蛋白（DF 3抗原）是有效的肿瘤标记物。DF 3抗原的唾液酸寡糖表达与食管鳞癌预后不良有关.腺癌（1）即使在胰腺和肝内胆管的扩张生长型肿瘤中，肿瘤的侵袭性生长区域也获得了在侵袭性生长型肿瘤中常见的MUC 1粘蛋白表达的特征。(2)原位杂交研究表明，MUC 2在胃癌中的表达与胃癌的发生、发展密切相关。 ...更多信息 大部分胰腺和肝内胆管扩张型肿瘤受MUC 2 mRNA调控，MUC 2 mRNA表达阳性者生存率明显高于MUC 2 mRNA表达阴性者。(3)MUC 5AC（胃型）和MUC 2（肠型）基因表达的模式在“胰腺粘液生成肿瘤（MPTs）”，这通常揭示较少的侵入性生长，显示MPTs亚型之间的变化。(4)在胰腺和肝内胆管肿瘤中，MUC 1粘蛋白的唾液酸表位在肿瘤的侵袭生长中起重要作用。(5)在壶腹癌和胃癌中，MUC 1（+）表达的患者预后差于MUC 1（-）表达的患者，而MUC 2（+）表达的患者预后好于MUC 2（-）表达的患者。(6)结肠直肠上皮肿瘤中腺周成纤维细胞鞘的显著减少反映了腺瘤-癌序列的进展。少

项目成果

Tabata M., Sugihara K., Yonezawa S., Yamashita S., Maruyama I.: "An immunohistochemical study of thrombomodulin in oral squamous cell carcinoma and its association with invasive and metastatic potential." Journal of Oral Pathology & Medicine. 26. 258-264

Tabata M.、Sugihara K.、Yonezawa S.、Yamashita S.、Maruyama I.：“口腔鳞状细胞癌中血栓调节蛋白的免疫组织化学研究及其与侵袭和转移潜力的关系。”

Sakamoto H., Yonezawa S., Utsunomiya T., Tanaka S., Kim Y.S., Sato E.: "Mucin antigen expression in gastric carcinomas of young and old adults." Human Pathology. 28. 1056-1065 (1997)

Sakamoto H.、Yonezawa S.、Utsunomiya T.、Tanaka S.、Kim Y.S.、Sato E.：“年轻人和老年人胃癌中的粘蛋白抗原表达。”

Sagara M,et al.: "Expression of mucin 1(MUC1)in esophageal squamous cell carcinoma:Its relationship with prognosis of the patients" International Journal of Cancer. (印刷中).

Sagara M 等人：“食管鳞状细胞癌中粘蛋白 1 (MUC1) 的表达：其与患者预后的关系”《国际癌症杂志》（出版中）。

Osaka M,et al.: "Immunohistochemical studies of mucin antigens in pancreas and intrahepatic bile-duct tumors" J.Hep.Bil.Pancr.Surg.4. 149-156 (1997)

Osaka M,et al.：“胰腺和肝内胆管肿瘤中粘蛋白抗原的免疫组织化学研究”J.Hep.Bil.Pancr.Surg.4。

Itoh T,et al.: "Expression of mucin antigens and LeX-related antigens in carcinomas and dysplasia of the pharynx and larynx" Recent Advances in Gastroenterological Carcinogenesis. I. 785-789 (1996)

Itoh T 等人：“咽和喉癌和发育不良中粘蛋白抗原和 LeX 相关抗原的表达”胃肠癌发生的最新进展。