Development of novel diagnosis method for minutecancer by infrared fluorescence endoscopy using fluorescence-emitting antibody by infrared ray excitation.

开发利用红外线激发的荧光发射抗体的红外荧光内窥镜诊断微小癌症的新方法。

• 批准号: 08457168
• 负责人: ITO Susumu
• 金额: $ 5.12万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457168/
• 关键词: ICG Delivative; Endoscopic Diagnosis; Fluorescence Marker; Infrared Fluorescence; Infrared Fluorescenct Endoscopy; Vital Immunohistochemical staining

ICG-sulfo-OSu (ICG N-hydroxy sulfo succinimide ester), as the labeling material that is fluorescent on excitation with infrared ray(IR) (Bioorg Med Chem Letters 1995 ; 5 : 2689-94, Bioorganic &Medicinal Chemistry 6(11) : 2179-2184, 1998) and the imaging system for IR fluorecent microscope(Dig Endosc 1997 ; 278-82) were developed in this experiment. The intensity of fluorescence of ICG-sulfo-OSu is too low for detection of microlesions by IR imaging system. Therefore, in the present study, we developed an imaging system using a highly sensitive charge-coupled-device (ICCD) (Digestive Endoscopy 11, 1999 (in press)) and the reinforcement agents (Bioorganic &Medicinal Chemistry 6(5) : 613-618, 1998) that can be used to image this labeling substance at the tissue level. Therefore, provide the fact that some antibodies which are specific to cancer cells can detect cancer cells by IR fluorescence also by using such antibodies bound with ICG.(J Gastroenterol 1998 ; 33 : 467-71. J.Medical Investigation 46, 1999 (in press), Digestive Endoscopy 11, 1999 (in press))

本实验研制了ICG-磺酸-OSU(ICG-N-羟基磺基琥珀酰亚胺酯)作为红外线(IR)激发下的荧光标记材料(Bioorg Med Chem Letters 1995；5：2689-94，BioOrganic&Medical Chemical 6(11)：2179-2184,1998)和红外荧光显微镜成像系统(Dig Endosc 1997；278-82)。ICG-Sulo-OSU的荧光强度太低，无法用红外成像系统检测到微小病变。因此，在本研究中，我们开发了一种使用高灵敏度电荷耦合器件(ICCD)(消化内窥镜11,1999(印刷中))和增强剂(生物有机和药物化学6(5)：613-618,1998)的成像系统，可以用于在组织水平上对这种标记物质进行成像。因此，提供了这样一个事实，即一些针对癌细胞的抗体也可以通过使用与ICG结合的这种抗体来通过IR荧光检测癌细胞(J Gasterenterol 1998；33：467-71)。医学调查46,1999(在印)，消化内窥镜检查，11,1999(在印))

项目成果

Taoka S: "Reflected Illumination-Type Imaging System for the DevelopmenInfrared Fluorescence Endoscopy." Digestive Endoscopy. Vol.11 in press. (1999)

Taika S：“用于开发红外荧光内窥镜的反射照明型成像系统。”

Hirata T, Kogiso H, Morimoto K, Miyamoto S, Taue H, Sano S, Muguruma N,Ito S, Nagao Y: "Synthesis and reactivities of 3-indocyanine-green-acyl-1, 3-thiazolidine-2-thione (ICG-ATT) as a new near-infrared fluorescent-labeling reagent." Bioorganic & Medicina

Hirata T、Kogiso H、Morimoto K、Miyamoto S、Taue H、Sano S、Muguruma N、Ito S、Nagao Y：“3-吲哚菁-绿-酰基-1, 3-噻唑烷-2-硫酮的合成和反应性（

Ito S: "Development of an imaging system using fluorescent labeling substances excited by infrared rays." Digestive Endoscopy. Vol.9 No.4. 278-282 (1997)

Ito S：“利用红外线激发的荧光标记物质开发成像系统。”

N Muguruma: "Development of labeled antibodies using fluorescence emitting substance upon excitation with infrared rays" Journal of Gastroenterology. (in press).

N Muguruma：“在红外线激发下使用荧光发射物质开发标记抗体”胃肠病学杂志。

Muguruma N, Ito S, Hayashi S, Taoka S, Kakehashi H, Ii K, Shibamura S, Takesako K: "Antibodies labeled with fluorescence-agent excitable by infrared rays." J Gastroenterol. 33 (4). 467-471 (1998)

Muguruma N、Ito S、Hayashi S、Taoka S、Kakehashi H、Ii K、Shibamura S、Takesako K：“用可被红外线激发的荧光剂标记的抗体。”