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Physiological and pathological roles of myelin-associated oligodendrocytic basic protein (MOBP) in myeklin of central nervous system.

髓磷脂相关少突胶质细胞碱性蛋白（MOBP）在中枢神经系统髓磷脂中的生理和病理作用。

基本信息

批准号：
08457190
负责人：
SAKODA Saburo
金额：
$ 3.52万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

One of the structural features that characterize the central nervous system (CNS) myelin is the existence of the radial component, which has been described as a junctional complex in a wide phylogenetic range of animals, and runs radially and zigzaging through the myelin sheath. The biochemical composition, however, remains unknown. We previously reported that myelin-associated oligodendrocytic basic protein (MOBP) was abundantly expressed specifically in oligodendrocytes at the mRNA level, only next to myelin basic protein (MBP) and proteolipid protein (PLP) in rat, and shared several characteristics with MBP.We show here that MOBP was also enriched in the radial component-enriched myelin fraction, suggesting that MOBP was associated with the radial component. In MOBP-deficient mice, the compact myelin was formed, but the radial component was straight with a narrower space between two adjacent radial components. These observations combined with the freeze-fracture examination suggested that MOBP,cytoplasmic constituent of the radial component, was anchored tightly to the extracellular constituent of the radial component at some interval, and changed the course of the radial component from straight radial direction to well-organized oblique zigzag one. Furthermore, the myelin from MOBP-deficient mice exposed to hexachlorophene, known as a dysmyelinating agent, showed widening of the major dense lines. We conclude that MOBP is essential for normal organization of the radial component, and maintains the integrity of the myelin sheath.

表征中枢神经系统（CNS）髓磷脂的结构特征之一是存在放射状组分，其已被描述为在广泛的动物系统发育范围中的连接复合物，并且放射状地延伸并穿过髓鞘。然而，生物化学成分仍然未知。我们以前的报告，髓鞘相关的少突胶质细胞碱性蛋白（MOBP）是丰富的具体表达在少突胶质细胞在mRNA水平上，只有旁边的髓鞘碱性蛋白（MBP）和蛋白脂质蛋白（PLP）在大鼠，并共享一些特性与MBP。我们在这里显示，MOBP也富集在径向成分富集的髓鞘馏分，表明MOBP与径向成分。在MOBP缺陷型小鼠中，形成了致密的髓鞘，但放射状成分是直的，两个相邻的放射状成分之间的空间较窄。这些观察结合冷冻断裂检查表明，MOBP，径向组件的细胞质成分，在一定的时间间隔内紧密锚定的径向组件的细胞外成分，并改变了径向组件的过程从直线径向方向的组织良好的斜曲折之一。此外，MOBP缺陷小鼠暴露于六氯酚（一种髓鞘形成障碍剂）后，其髓鞘的主要致密线变宽。我们的结论是，MOBP是必不可少的径向组件的正常组织，并保持髓鞘的完整性。