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Cloning of disease-causing genes for the syndrome with congenital heart malformations

先天性心脏畸形综合征致病基因的克隆

基本信息

批准号：
08457231
负责人：
MINOSHIMA Shinsei
金额：
$ 4.29万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457231/
关键词：
congenital heart malformations cat eye syndrome marker chromosome region specific cosmid library BAC library BAC cosmid contig genomic sequencing gene exon prediction 領域特異的コスミドライブラリーコスミドコンティグゲノミックシーケンシング猫眼症候群コスミドライブラリー

项目摘要

We have approached the disease-responsible genes for heart malformation in cat eye syndrome (CES) and obtained the following results.(1) We have constructed BAC/cosmid contigs of the CES chromosomal region (CER ; 1.9 Mb in size). We used two DNA libraries, a CES region-specific cosmid library (9,200 clones ; redundancy, 10) from the flow-sorted marker chromosomes of a CES cell line CH91-157 and a BAC library (200,000 clones ; redundancy, 7.5) from human total DNA, both of which were established by ourselves. A number of known DNA markers, newly established STS's from cosmid clones, and YAC clones isolated with these DNA markers were used to screen the BAC and CES cosmid libraries, and as a result 1,740 cosmids and 116 BAC clones were isolated. The vectorette PCR method and fingerprinting have been applied to construct BAC/cosmid contigs. To date, 6 contigs consisting of 33 BAC and 43 cosmid clones have been constructed to cover >1.3 Mb region.(2) Genomic sequencing of 4 BAC and 7 cosmid clones have almost been finished which covers 〜300 kb in CER. We have encountered a great difficulty to construct contigs and determine the sequence of this peri-centromeric region due to high contents of repetitive sequences and high homology with other chromosomes such as chromosome 14 and other heterochromatin-containing chromosomes.(3) The DNA sequence is being analyzed by exon-prediction programs (GENSCAN, GRAIL) and homology search tools (BLAST, FASTA) followed by isolation of corresponding cDNA's.

我们已经接近了猫眼综合征（CES）心脏畸形的致病基因，并获得了以下结果。(1)我们构建了CES染色体区域的BAC/cosmid contigs （CER， 1.9 Mb大小）。我们使用了两个DNA文库，一个是来自CES细胞系CH91-157的流分类标记染色体的CES区域特异性cosmid文库（9200个克隆，冗余10个），另一个是来自人类总DNA的BAC文库（200,000个克隆，冗余7.5个），这两个文库都是我们自己建立的。利用一些已知的DNA标记、从cosmid克隆中新建立的STS标记以及用这些DNA标记分离的YAC克隆对BAC和CES cosmid文库进行筛选，最终分离出1,740个cosmid和116个BAC克隆。应用载体PCR法和指纹图谱技术构建了BAC/cosmid序列。迄今为止，已经构建了6个contigs，包括33个BAC和43个cosmid克隆，覆盖了bb0 1.3 Mb的区域。(2) 4个BAC和7个cosmid克隆的基因组测序已基本完成，测序面积约为300kb。由于重复序列的高含量和与其他染色体（如14号染色体和其他含异染色质的染色体）的高度同源性，我们在构建contigs和确定该着丝粒周围区域的序列时遇到了很大的困难。(3)利用外显子预测程序（GENSCAN、GRAIL）和同源性搜索工具（BLAST、FASTA）对DNA序列进行分析，并分离相应的cDNA。