In vitro model of Brain-Blood Barrier using cultured endothelial cells, astrocytes and extracellular matrices

使用培养的内皮细胞、星形胶质细胞和细胞外基质的脑血屏障体外模型

• 批准号: 08457360
• 负责人: TAKEUCHI Shigekazu
• 金额: $ 4.8万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457360/
• 关键词: Blood-Brain barrier; Endothelial cell; Astrocyte

The interaction of endothelial cells and astrocytes was investigated in vitro.Microendothelial cells from Fisher's rat brain and astrocytes from new born Fisher's rat were used in this study. The endothelial cells were cultured on a filer coated with type 1 collagen, and treated with fibronectin, and astrocytes were cultured on the reverse side of the filter. Type 1 collagen and fibronectin stimulated the growth and differentiation of the endothelial cells. The endothelial cells co-cultured with astrocytes showed tight junctions between the cells and positive activity for gamma - GTP, although the number of tight junction was small, and the activity of gamma - GTP was low.Endothelial cells were incubated in type 1 collagen, and capillary formation was successfully made. Condioned media derived from the culture was applied to the co-cultured system, and the system was incubated for a few days. The conditioned media had no effect on the number of tight junction and the activity of gamma - GTP.

本研究采用Fisher’s大鼠脑微内皮细胞和新生Fisher’s大鼠星形胶质细胞，在体外研究了内皮细胞与星形胶质细胞的相互作用。内皮细胞在涂有1型胶原的滤器上培养，并用纤连蛋白处理，星形胶质细胞在滤器的反面培养。1型胶原和纤维连接蛋白刺激内皮细胞的生长和分化。与星形胶质细胞共培养的内皮细胞显示细胞间的紧密连接和γ- GTP活性，但紧密连接的数量较少，γ- GTP活性较低。将来源于培养物的条件培养基应用于共培养系统，并将该系统孵育几天。条件培养液对细胞紧密连接的数目和γ- GTP活性无影响。