The experimental study of long-term heart preservation with the combined method of simple immersion and coronary perfusion through the new technique for the evaluation of cardiac function using force transducer

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In our previous study, the donor heart was well preserved by the combined method of 12-hr simple immersion and 1-hr coronary perfusion. The efficacy of diluted blood was confirmed as perfusate for coronary perfusion compared with the University of Wisconsin (UW) solution. In addition, we designed an original apparatus for continuous coronary perfusion preservation instead of the Langendorff's method This apparatus has such characteristics as hypothermic management, variable perfusion pressure, non-dependent energy source and portable. We reported the usefulness of this apparatus in canine models through 12-hr preservation followed by orthotopic transplantation. We are now planning the evaluation of transplanted hearts by the force transducer and long-term heart preservation of more than 12-hr.